The largest single cave chamber in North America, the Big Room at Carlsbad Caverns, was once dubbed "The Grand Canyon with a roof" by Will Rogers. Visitors can take an elevator or walk the winding mile-plus Natural Entrance Trail down to the massive limestone enclosure, where multiple trails...
F-12 nutrient mixture (Ham) containing 1 mm l-glutamine was purchased from Invitrogen (Carlsbad, CA). Whatman flexible plates for TLC (AL SIL G; 20 × 20 cm; aluminum backing; silica gel coating, 250 µm layer) were obtained from Fisher Scientific (Pittsburgh, PA). ...
Minimum essential medium-alpha (α-MEM) was purchased from Gibco, Carlsbad, CA, USA, and Ex-Cyte from Millipore, Billerica, MA, USA. Insulin (3.5 mg [100 IU]/mL) was obtained from Novo Nordisk, Søborg, Denmark. MTT dye, collagenase type I, and TRIZOL (TriQuick, Catalogue #R1100) ...
PCR assays were performed using 5 μL of the DNA samples in a mixture containing 0.75 U Platinum Taq DNA Polymerase (Invitrogen, Carlsbad, CA, USA), PCR buffer (PCR buffer 10 × 100 nM Tris-HCl, pH 9.0, 500 mM KCl), 0.2 mM deoxynucleotides (dATP, dTTP, dCTP and dGTP) (Invitrogen...