The best answer is that CaCl2 is neither basic nor acidic (it's a salt). If you were pressed to explain why the CaCl2 solution would be "slightly basic" then you can "rationalize it" I suppose by the fact that for every one calcium ion that is released you release two chloride ions....
CI-MPR binds ligands in a pH-dependent manner, interacting optimally at the pH of the Golgi (~ pH 6.8), retaining binding ability at the cell surface, and releasing its cargo in the acidic environment of late endosomes6 (Fig. 1A). The 15 domains that comprise CI-MPR’s ~ 2300...
implying that lactate and/or pyruvate release from OLs has a role in axonal health9,13. Glutamatergic signaling has been shown to stimulate the surface expression of glucose transporter 1 (GLUT1) in OLs, suggesting that axonal activity might regulate the metabolic support provided by OLs to axons...
a,bHEK 293T cells expressing mouse LDLR, LDLRAD3 or TIMD4 or transfected with pCAGGS vector (negative control) were infected with the rGETV-EGFP. At 24 h post infection viral RNA (vRNA) levels in infected cells were measured via qRT-PCR (normalized relative to WT cells) (a) and v...
components, vGlut-1, VAMP-2, and Synaptotagmin I, ~50–60% of the tagged protein appears in a recycling pool that responds readily to sustained action potential stimulation by mobilizing and fusing with the plasma membrane, while the remainder is targeted to a nonrecycling, acidic compartment. ...
We found that the intensity of the cross-linked VDAC antibody-labeled protein bands was highly increased following treatment of the blot with acidic pH conditions (0.1 M glycine, pH 2.2–4.0). Therefore, in all subsequent experiments, such treatment was performed prior to immunoblotting. The ...
2H) as water-soluble products of LPI cleavage eliminated the possibility of GDE3 being a lysophospholipase D or A, respectively. The GDE3 mechanism differed from that of bacterial PLC, which produced cyclic Ins-1,2-phosphate (Fig. 2I). This was partially converted into Ins1P upon acidic ...
(Kate) Drummond MBBS, PhD, works as a neurosurgeon at Royal Melbourne Hospital, simultaneously conducting basic research into the mechanisms of glioma invasion. She heads the Central Nervous System Tumours at Royal Melbourne Hospital and is a national leader in surgical neuro-oncology and executive ...
sections, staining for various cell-type markers revealed that the dNP2-dTomato protein signal was significantly co-localized with neurons (Neuronal Nuclei (NeuN)-positive cells), astrocytes (Glial fibrillary acidic protein (GFAP)-positive cells) and microglial cells (Ionized calcium binding adaptor ...
Altogether, this indicates that acidic pH-dependent endosomal proteases are involved in NTF generation. We also assessed the impact of the broad-spectrum serine and/or cysteine protease inhibitors AEBSF (Supplementary Fig. 2e, f), E-64d and leupeptin (Supplementary Fig. 2g, h), which influenced...