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C2C12 cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Sigma-Aldrich M5655). After the addition of MTT to the medium, the optical density was determined at 570 nm using a microplate reader. To separate nuclear proteins, NE-PER ...
a Strong immunostaining of TMED3; b moderate staining of TMED3; c weak staining of TMED3; d negative staining of TMED3 in breast cancer tissue; e negative staining of TMED3 in normal breast tissue; f moderate staining of TMED3 in the duct of normal breast tissue. Scale bar stands for ...
Telomere Restriction Fragment (TRF) images signal was extracted using ImageQuant (GE Healthcare) 1D gel analysis mode and manual lane creation. Ladder signal intensities were extracted from Ethidium bromide gel images, setting the lane upper limit at the gel wells bottom. Pixel position of ladder ...
[00:55:27]Mike Rowe:Follow your passion as a bromide is precisely what 98 percent of the people who audition forAmerican Idol.And they're lined up. Thousands of people who have been told if you believe something deeply enough and if you want something bad enough, and if you truly embrac...
To avoid cell overgrowth, different numbers of cells were seeded in a 96-well plate (Sarstedt, Germany): 5,000 for A549 and 10,000 for MCF7 and HT29 cells. After 48 h growth, 10 µL of 5 g/L MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, Thermo Fisher ...
also became strong also believed in tran also brings a green also building the str also called fenyang v also called natal yea also crops up also decline steadily also diligent also for also for newborn babi also have good also known as chestnu also love shandong me also magical also not...
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Innate immune responses are evolutionarily conserved processes that provide crucial protection against invading organisms. Gene activation by potent NF-κB transcription factors is essential both in mammals and Drosophila during infection and stress chal