The histone-like nucleoid structuring (H-NS) protein is a DNA binding factor, found in gammaproteobacteria, with functional equivalents in diverse microbes. Universally, such proteins are understood to silence transcription of horizontally acquired genes
To delve deeper into the functionality of dominant lineage-specific genes, we explored their expression patterns across both environmental and infection conditions. We selected representative strains - “K96243” (lineage 1 – sub-lineage 1.1), “UKMD286” (lineage 2 – sub-lineage 2.1), and “...
falciparum blood-stage growth creates significant oxidative stress that can cause oxidative damage to both host and para- site lipid membranes. Interestingly, P. falciparum has been shown to internalize host antioxidant enzymes such as catalase, supe...
Nodules may ulcerate and drain small amounts of pus or blood-tinged fluid. The differential diagnosis should include the bacterial and fungal causes of lymphangitis (glanders, equine histoplasmosis, ulcerative lymphangitis) and sterile nodular panniculitis (q.v.). Diagnosis is made by cytology,...
Among these proteins were the proteoglycan biglycan (BGN), which is typically found in the extracellular matrix of blood vessels [75], and Endothelial Cell–Specific Molecule 1 (ESM1), also known as endocan. Both proteins play roles in regulating endothelial cell function and angiogenesis, and ...
Extent of differential allelic expression of candidate breast cancer genes is similar in blood and breast. Breast Cancer Res. 2009;11(6):R88. Article Google Scholar Taylor SC, Laperriere G, Germain H. Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from...
Intriguingly, we discovered KU-177, an AHSA1 selective inhibitor, and found KU-177 targeting the same site as Bufalin. Bufalin and KU-177 treatments hampered the proliferation of flow MRD-positive cells in both primary MM and recurrent MM patient samples. Moreover, KU-177 abrogated the ...
Upon analysing a panel of six ovarian cancer cell lines in response to PARGi and the PARP inhibitor Olaparib, hereafter PARPi, we discovered that OVSAHO, COV318, COV362 and CAOV3 proliferated in both inhibitors, while Kuramochi and OVCAR3 displayed differential sensitivities; Kuramochi ...
Intact sacculi (untreated), sacculi subjected to mild acid hydrolysis (mild acid), or deamination with HONO (HONO) were incubated with GFP-AtlAEfs, and examined by fluorescence microscopy (GFP-AtlAEfs, top panels) and differential interference contrast (DIC, middle panels). An overlay of fluore...
Differential centrifugation of lysates of FDMs untreated (control) or treated with 50 mM etoposide for 16 h (ca. 50% cell death), using the lysosomal purification kit from Sigma. Each fraction was assayed for protein concentration and acid phosphatase activity, (a) as well as anti-Lamp-1, ...