Cells need to efficiently process replication-associated recombination structures to prevent chromosome mis-segregation during mitosis. To do so, they rely on the action of structure-specific endonucleases, such as Mus81–Mms4 and Yen1, and on the Sgs1-Top3-Rmi1 (STR) dissolvase complex to pr...
Under a Creative Commons license open accessHighlights Summary Programmed cell death is a pivotal process that regulates neuronal number during development. Key regulators of this process are members of the BCL-2 family. Using mRNA differential display, we identified a Bcl-2 family gene, Blm-s (...
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the...
https://doi.org/10.1016/j.jbc.2021.101494Get rights and content Under a Creative Commons license open accessChanging physiological conditions can increase the need for protein degradative capacity in eukaryotic cells. Both the ubiquitin-proteasome system and autophagy contribute to protein degradation. How...
Taken together, these data highlight an important biochemical link between the UbS-DDR and BLM-dependent pathways involved in maintaining genome stability. 展开 关键词: RAP80 RNF8 RNF168 K63-linked ubiquitylation PML nuclear bodies DOI: 10.1038/emboj.2013.117 被引量: 35 年份: 2014 ...
Specifically, the expression of CA9, CDC42EP5, VEGFB, TNFRSF4 and DDR1 was downregulated following miR-876 treatment, whereas expression of BRCA2 and BLM was upregulated. The differential expression of several of these genes was confirmed using qRT-PCR analysis in treated tumors (Fig. 4D) ...
RecQ helicases play an essential role in the maintenance of genome stability. In humans, loss of RecQ helicase function is linked with predisposition to cancer and/or premature ageing. Current data show that the specific depletion of the human RECQ1 heli
Ideally, however, the question whether there is a raise in the prevalence of MIH would be answered by studies assessing children of the same age group after a considerable time span of several years under standardized and well-calibrated conditions. Therefore, in a separate analysis on data from...
Saline: n = 6, BLM: n = 8. (B) The mRNA expression of Dbn1 in macrophages (Mac) or myofibroblasts (Myo) isolated from mouse lungs at 14 days after bleomycin administration. Total RNA extracted from macrophages or myofibroblasts was subjected to real-time RT-PCR. ∗∗∗P < 0.001,...
Briefly, the protein was expressed in Escherichia coli BL21(DE3) from a pGEX-6P vector as a GST-fusion protein at 37 °C under standard conditions; hSSB2-OB was purified using glutathione affinity chromatography, HRV3C protease cleavage and size exclusion chromatography as the final purification ...