People have been using Biotin for decades. Whether to grow their hair, thicken their nails, or boost their metabolism, Biotin has become a trusted and reliable source for the health conscious everywhere. What people don’t know is how many benefits this vitamin actually has! Internal Uses of ...
. We found that lordosis behavior was not affected in (OVX+E+P)GnRH::Cre; DicerloxP/loxPfemales (Mann–WhitneyUtest;P = 0.79; Fig.4b). These results suggest that although GnRH neurons are required for mate preference, they might not be essential for the expression of lordosis behavior...
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for protein–protein interactions. Thus, the N-terminal portion of SPEM1 (~28 a.a.) was synthesized and purified. A biotin was added onto the C-terminus of this peptide. The biotin-labeled N-terminal 28 a.a. peptides of SPEM1 were attached to streptavidin beads, which were then ...
A 521-bp SHBG-(628–1148) biotinylated probe was prepared by PCR incorporation of biotin-14-dCTP (Invitrogen) into the SHBG PCR product. The probe was added to the slides, which were heated (95 °C) for 10 min, and then incubated overnight (37 °C) in a humidified chamber. Slides ...
Secondary antibodies biotinylated goat anti-rabbit or anti-mouse IgG (Vector, 1:200) were diluted in PBS containing 0.25% Triton-X-100, followed by incubation with the avidin/biotin complex-HRP (VECTASTAIN Elite ABC Kit Standard, Vector Laboratories, CA, USA) for 30 min. Finally, the ...
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Aim: Severe biotin deficiency associated with biotinidase enzyme deficiency in newborns is seen as severe neurological problems and hearing loss. However, ... Yllmaz, Nevreste Didem SonbayGur, Ozer ErdemEnsari, NurayBulut, ErdoganKaya, Ozlem TugceSirvanci, SerapDanisman, BetulDerin, NarinGezgin,...
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