Bioluminescence images were acquired 5 min after luciferin administration on an IVIS200 system (Perkin Elmer, Waltham, MA, USA). Bioluminescence signal was quantified using the LivingImage software (Perkin Elmer
2C). The results show that selenite (at the concentration used) induced VDAC oligomerization when most cells (85%) were in the early apoptotic state. Next, to directly monitor the oligomeric status of VDAC1 molecules in the membranes of living cells, the bioluminescence resonance energy transfer...
Application of bioluminescence imaging (BLI) to the study of the animal models of human infectious diseases. Chapter in BR Moyer et al. Pharmaco-imaging in drug and biologics development. American Association of Pharmaceutical Sciences, 2014. Google Scholar Griffin, 2002 Griffin JFT. A strategic ...
ER- positive or TN breast tumours (N = 6) were implanted in the fat pad of NOD/SCID immunodeficient mice, and collected when reaching 1 cm diameter, as previously described [7, 29]. Bioluminescence and microcomputed tomography (micro-CT) imaging Experiments were conducted as previously ...
Bioluminescence was assessed with 100 μM coelenter- azine (sc-205904, Santa Cruz Biotechonolgy) prior diluted in PBS supplemented with 5 mM NaCl, at pH 7.2 and stabilized at room temperature for 30 min. In short, 5 μL serum per sample were transferred into Nunc™ F96 MicroWell™ ...
structured subdomains of DVL proteins18. The isolated DEP domain interacts strongly with overexpressed FZDs in a constitutive and phosphatidylinositol(4,5)bisphosphate (PIP2)-dependent manner, which can, for example, be assessed using microscopy- or more recently also bioluminescence resonance energy tra...
Yongjian money and the phenomenon of bioluminescence research In 1994, Chinese-American scientists Yongjian money (Roger Y Tsien) began to transform GFP, a number of discovery. In the world with the most money Yongjian laboratory modified variant, and some stronger fluorescence, some yellow, blue,...
All the cells were luciferase labeled to facilitate Bioluminescence imaging (BLI) in vivo. To investigate whether loss of SMARCB1 promotes BLCA disease progression, we used an orthotopic mouse NOD/SCID/IL2rγ- null (NSG) model wherein luciferase labeled T24 human bladder can- cer cells (control...
In these mice, activation of KrasG12D in the lung epithelium via intranasal instillation of adenovirus expressing Cre-recombinase (Ad-Cre) is coupled to simultaneous deletion of Prkaa1 and Prkaa2 and activation of firefly luciferase to allow for noninvasive bioluminescence imaging (BLI) of NSCLC ...
. Total ATP levels from 1 × 105 cells were measured using an ENLITEN ATP Assay System Bioluminescence Detection Scientific Reports | 5:15798 | DOI: 10.1038/srep15798 12 www.nature.com/scientificreports/ kit (Promega) according to the manufacturer's protocol. The luminescence intensity ...