Is pBR322 a shuttle vector? Most expression vectors for extrachromosomal protein expression and shuttle vectors contain the pBR322origin of replication, and fragments of pBR322 are very popular in the construction of intraspecies shuttle or binary vectors and vectors for targeted integration and excisio...
CONCLUSION: A binary adenoviral vector system provides an effective approach to amplify viral vectors that express potentially toxic gene, TRAIL. Ad/GT-TRAIL showed a significantly enhanced therapeutic efficiency for HT29 when coinfected with Ad/PGK-GV16. Ad/GT-TRAIL could induce apoptosis of HT29...
A similar binary start-site mechanism has been described for other proteins dual-localized to the cytosol and mitochondria (44). To determine the subcellular distribution of Mxr1 and Mxr2 in yeast, we fractionated lysates prepared from cells expressing GFP-tagged Mxr1 or Mxr2 proteins. Lysates ...
To successfully invade new hosts, plant viruses must break host resistance and be competent to move within and between plant cells. As a means, viral proteins known as pathogenicity determinants have evolved to coordinate a network of protein interaction
PAA represents a time series T of length n in a w-dimen- sional space by a vector of real numbers, T¯ = t¯1, . . . , t¯w. The ith element of T¯ is calculated by the equation: t¯i = w n n w i Tj j = n w (i −1) + 1 3 2 A time series T PAA...
p35S-ACA2-GFP Plant TDNA binary vector expressing the ER marker ACA2p Ref. 31 AtSTE24 The protein data base was searched for a plant homologue of STE24 using the yeast and human STE24 amino acid sequences. A genomic sequence from Arabidopsis thaliana(GenBankTM accession number AF007269)...
A simple example of cache-friendly versus cache-unfriendly is c++'sstd::vectorversusstd::list. Elements of astd::vectorare stored in contiguous memory, and as such accessing them is much more cache-friendly than accessing elements in astd::list, which stores its content all over the place. ...
The main purpose of the system is to hijack the in-game 3D camera by overwriting values in its camera structure with our own values so we can control where the camera is located, it's pitch/yaw/roll values, its FoV and the camera's look vector. [250星][2m] [Py] hacktoolspack/...
ADP, thus generating cytosolic ATP (Extended Data Fig.10a). This PCr shuttle provides readily available ATP for energy-consuming processes throughout the rest of the cell36,37. To deplete PCr from the cytosol, we treated BMDMs with cyclocreatine (cyCr)—a creatine analog. Cyclocreatine is ...
PVX-mV2 has a nonsense mutation in the N-terminus region, and PVX-V2ΔN having 58 aa deletion at the N-terminus, while PVX-V2ΔC contains a deletion of 58 aa at the C-terminus of ToLCJV-A[ID]. For transient expression, binary vector pBI121 was used. LB is the T-DNA left ...