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Response to: "Is an increase in duodenal bicarbonate concentration after STa really enhanced bicarbonate ion secretion?". A response by Zachary M. Sellers et al. to a letter to the editor about their article "Heat-Stable Enterotoxin of Escherichia coli (STa) Can Stimulate Duod... Sellers,M ...
Bicarbonate ion transport by the electrogenic Na+/HCO3 cotransporter, NBCe1, is required for normal electrical slow﹚ave activity in mouse small intestinegastrointestinal motilityinterstitial cells of Cajalsmall intestinemousesmooth musclebiological pacemakersSlc4a4 protein...
Match between runs was employed and the significance threshold of the ion score was calculated based on a false discovery rate of < 1%. MaxQuant normalized ratios were analyzed as follows: for a total of p unique kinases, we computed the pooled protein ratio and p-value across the ...
bicarbonate therapy and hemodialysis through strict control of D-lactate production10. However, there is no treatment for symptoms specifically caused by LDHD deficiency. In addition, LDHD mutations also lead to elevated levels of other 2-hydroxyacids such as D-2-hydroxyisovalerate, D-2-hydroxyiso...
Exact balance from an acid-base perspective is achieved with a crystalloid strong ion difference of 24mEq/l. This can be done simply by replacing 24mEq/l of chloride in 0.9% sodium chloride with bicarbonate or organic anion bicarbonate substitutes. Potassium, calcium and magnesium additives are ...
The sample was diluted to 0.6 M final Gn-HCl concentration with 25 mM ammonium bicarbonate buffer. For digestion of protein, trypsin was added in a trypsin-to-lysate ratio of 1:50 after and incubation was performed overnight at 37 °C. The supernatant was vacuum dried and the peptides ...
The mass spectrometer was operated in positive ion mode. MS/MS spectra were acquired with either higher-energy collisional dissociation (HCD) in Orbitrap (Q-Exactive) or collision-induced dissociation (CID) in ion trap (Fusion Lumos). The spectra labeling, database search, and quantification were...
A sample of recombinant LafrOBP1 (20 μg) was dissolved in 0.1 M tetraethylammonium bicarbonate (TEAB), pH 6.5, containing 4 M guanidinium chloride, and then treated with iodoacetamide (0.5 M final concentration) for 30 min, in the dark. The protein was precipitated by the addition of 6 ...