2007. Is bicarbonate buffer suit- able as a dissolution medium? J Pharm Pharmacol 59(10):1375-1382.Boni, J.E., R.S. Brickl, and J. Dressman, Is bicarbonate buffer suitable as a dissolution medium? J Pharm Pharmacol, 2007. 59(10): p. 1375-82....
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(Nikon, Japan) in a perfusion chamber at room temperature. The inverted microscope was coupled with a dual-wavelength excitation spectrofluorometer (Intracellular imaging, USA). Cells were perfused with Krebs’ bicarbonate buffer (KB buffer, mM; 117 NaCl, 5 KCl, 1.2 MgSO4, 1.2 KH2PO4, 1.25 ...
Briefly, active fractions were reduced by incubation at 57˚C for 30 min with 10 mM 1,4-dithiothreitol (DTT) and 10 mM ammonium bicarbonate buffer, alkylated with 50 mM of indole-3-acetic acid (IAA) at room temperature for 45 min without light access, and then digested overnight at ...
100 µl/well of 2 µg/ml K8.1 or ORF73 diluted in 0.05 M carbonate/bicarbonate buffer, pH 10 or 1 × PBS respectively was incubated in plate wells overnight at 4 °C. The plates were washed three times with PBS-T and then blocked with 280 µl/well of assay buffer [2.5...
Parasitized blood from these animals was diluted in Krebs-Ringer bicarbonate buffer (Sigma-Aldrich, St. Louis, MO) and normal saline to deliver 105 iRBCs i.p. in 200 μl into experimental WT or IL-10 KO mice. Thin blood smears were collected at regular intervals to monitor for peripheral...
The sample was diluted to 0.6 M final Gn-HCl concentration with 25 mM ammonium bicarbonate buffer. For digestion of protein, trypsin was added in a trypsin-to-lysate ratio of 1:50 after and incubation was performed overnight at 37 °C. The supernatant was vacuum dried and the peptides ...
Group A, vaccinated by oral gavage, with 5 × 107 CFU of S. Typhimurium ΔznuABC dissolved in 20 ml of sodium bicarbonate buffer, and boosted after two weeks with an intramuscular administration of inactivated S. Choleraesuis at the dose of 2 × 109 CFU/ml. Group B was intramuscularly ...
Peak RER values continuously increased, but as activity recordings were not indicative of intense activity, an activator bicarbonate buffering which elevates the RER > 1 (Supplementary Fig. 1a), and given that this increase was present in time-restricted and ad libitum-fed animal, suggests a...
Cells were then washed twice with PBS and incubated in Krebs-Ringer Bicarbonate buffer (30 mM HEPES-NaOH pH 7.4, 10 mM NaHCO3, 120 mM NaCl, 4 mM KH2PO4, 1 mM MgSO4, 0.75 mM CaCl2) supplemented with 10 mM glucose and 0.5% BSA. Cells were incubated in the ...