Aminoglycoside: Class of antimicrobial antibiotic agents primarily used to treat gram-negative infections. In addition to the class leader streptomycin, neomycin, gentamicin, kanamycin, tobramycin, amikacin, and
OSCC cell line SCC-9 cells were cultured in DMEM/F12 with 10% FBS, 1% antibiotic-antimycotic, and 400 ng/mL hydrocortisone. 2.2. Plasmids The human FOXM1a, FOXM1b, or FOXM1c gene was obtained from CAL 27 cells by RT-PCR with primers 5 -ATGAAAACTAGCCCCCGTCG-3 and 5 -CTACTGTAG...
A phenotypic selectable marker is, for example, a gene encoding a protein that confers antibiotic resistance or that supplies an autotrophic requirement, and an origin of replication recognized by the host to ensure amplification within the host. Other useful expression vectors for prokaryotic host ...
shortened treatment regimen4,5,6. Unfortunately, these pre-clinical model end points did not translate to humans and in a recent clinical trial in which MOX replaced either ethambutol (EMB) or isoniazid (INH), MOX did not shorten the standard 6 month TB treatment to 4 months7. One conclusio...
CVCL_4U18) were cultured and sub-cultured in the standard maintenance medium, Dulbecco's modified Eagle's medium (DMEM): Ham's F12 (1:1; v/v) with 2 mM Glutamine, 12.5% fetal bovine serum (FBS), and 1% antibiotic/antimycotic mix, in accordance with the standard guidelines, procedures,...