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A phenotypic selectable marker is, for example, a gene encoding a protein that confers antibiotic resistance or that supplies an autotrophic requirement, and an origin of replication recognized by the host to ensure amplification within the host. Other useful expression vectors for prokaryotic host ...
shortened treatment regimen4,5,6. Unfortunately, these pre-clinical model end points did not translate to humans and in a recent clinical trial in which MOX replaced either ethambutol (EMB) or isoniazid (INH), MOX did not shorten the standard 6 month TB treatment to 4 months7. One conclusio...
(Thermo). The lipid–siRNA complex was then mixed with antibiotic-free RPMI. The prepared transfection media was added to the cells (500 µL per well) and incubated for 24 h at 37 °C. Twenty-four hours post-transfection, media was replaced with antibiotic-positive media and incubated ...
CVCL_4U18) were cultured and sub-cultured in the standard maintenance medium, Dulbecco's modified Eagle's medium (DMEM): Ham's F12 (1:1; v/v) with 2 mM Glutamine, 12.5% fetal bovine serum (FBS), and 1% antibiotic/antimycotic mix, in accordance with the standard guidelines, procedures,...