Petushkov, ATP is a cosubstrate of the luciferase of the earthworm Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae), Dokl. Biochem. Biophys. 392 (2003) 253-255.N. S. Rodionova,V. S. Bondar’,V. N. Petushkov.ATP Is a Cosubstrate of the Luciferase of the Earthworm...
The standard reaction system included 10 μg GhGLU18 recombinant protein dissolved in PBS buffer and 4 % (w/v) of β-glucan substrate dissolved in 200 μL sodium acetate (100 mM, pH 5.2). The entire reaction system was first incubated at 55°C for 10 minutes, then 500 μL DNS ...
Nutlin-3 is a potent inhibitor of the interaction between p53 and its negative regulator MDM223, thereby activating p53 to inhibit cell proliferation and tumorigenesis in models harboring wild-type TP53. As an assessment of potential drug synergy, we measured cell viability reflected in ATP levels ...
a Cartoon of the securin-separase fusion protein containing the full separase-binding region of securin (left) or with securin truncated on the C-terminal side of the pseudosubstrate motif (right). The separase active site (orange) and pseudosubstrate motif (red) are indicated. b Diagram of ...
What is substrate-level ATP formation?Question:What is substrate-level ATP formation?"Nuclear" Power:The A in ATP stands for Adenosine, an Adenine base bound to a ribose sugar, just like a nucleotide in RNA. The difference is that ribose is bound on the other to not one but three phospha...
V-ATPase is a large multisubunit complex composed of a peripheral domain (V1), responsible for hydrolysis of ATP, and an integral domain (V0) that carries out proton transport [15]. In sarcoma cells, the survival mechanism under acidic conditions and the activity of V-ATPase are still ...
这种方法揭示了内质网驻留的孤儿P型泵P5A-ATP酶(酵母中的Spf1;人类中的ATP13A1)直接与线粒体尾锚定蛋白相互作用。由于遗传学研究已经将P5A-ATP酶(P5A-ATPase)与线粒体尾锚定蛋白错位联系起来,这些作者结合生化和结构方法来确定P5A-ATP酶的功能和机制。 3. Result 这些作者在无细胞系统中重建了膜蛋白插入细胞...
ENSG00000105409.18 ATP1A3 –1.283429795 .035656 ENSG00000120885.21 CLU –1.280161277 5.36E–10 ENSG00000239264.8 TXNDC5 –1.260216411 .024028 ENSG00000035403.17 VCL –1.250574998 2.12E–18 ENSG00000147231.13 CXorf57 –1.243074389 .032543 ENSG00000166831.8 RBPMS2 –1.239626575 .032118 ENSG00000197168.12...
ATP-binding cassette (ABC) transporter proteins are efflux pumps that have relevance to multidrug resistance in nematodes and schistosomes [26]. A total number of 19 six-transmembrane helix ABC transporter domains (InterPro:IPR001140, Pfam: PF00664) were detected in E. multilocularis predicted gene...
ARTICLE Received 31 Mar 2016 | Accepted 22 Jun 2016 | Published 8 Aug 2016 DOI: 10.1038/ncomms12387 OPEN ATP-dependent substrate transport by the ABC transporter MsbA is proton-coupled Himansha Singh1, Saroj Velamakanni1, Michael J. Deery2, Julie Howard2, Shen L. Wei1 & Hendrik W. ...