Protein folding occurs while bound to the ATP-independent chaperone Spy Stull, F., Koldewey, P., Humes, J. R., Radford, S. E., and Bardwell, J. C. (2016) Substrate protein folds while it is bound to the ATP-independent chaperone Spy. Nature structural & molecular biology 23, 53-...
Here, we evaluate the role of the solvent-exposed residues of the translocation pathway in the process of substrate binding. We identify the main binding site, comprising three positively charged and a set of aliphatic and aromatic residues, which bind ADP and ATP in both states. Additionally, ...
Thus ATP production is not compromised in microglia from male APP/PS1 mice, which may provide an explanation for the maintenance of phagocytic function. PFKFB3 is a key regulator of glycolysis23,24 and an increase in expression of PFKFB3 in microglia is observed in glycolytic cells8,9,22. ...
They also provide compelling evidence that substrate proteins can fold while being continuously bound to a chaperone. 展开▼ 机译:伴侣分子协助细胞中许多蛋白质的折叠。尽管研究最深入的分子伴侣使用ATP结合和水解循环来辅助蛋白质折叠,但已发现许多分子伴侣在没有高能辅助因子的情况下会促进折叠。尚不清楚ATP...
ATP-binding cassette transporters mediate the transbilayer movement of a vast number of substrates in or out of cells in organisms ranging from bacteria to humans. Current alternating access models for ABC exporters including the multidrug and Lipid A tr
The proline-rich Akt substrate of 40 kilodaltons (PRAS40) was identified as a raptor-binding protein that is phosphorylated directly by mammalian target of rapamycin (mTOR) complex 1 (mTORC1) but not mTORC2 in vitro, predominantly at PRAS40 (Ser183). The binding of S6K1 and 4E-BP1 to ...
ENSG00000105409.18 ATP1A3 –1.283429795 .035656 ENSG00000120885.21 CLU –1.280161277 5.36E–10 ENSG00000239264.8 TXNDC5 –1.260216411 .024028 ENSG00000035403.17 VCL –1.250574998 2.12E–18 ENSG00000147231.13 CXorf57 –1.243074389 .032543 ENSG00000166831.8 RBPMS2 –1.239626575 .032118 ENSG00000197168.12...
Additionally, several proteins associated with metabolic pathways related to energy production (e.g. glycolysis, carbohydrate binding, conversion of ADP to ATP) were enriched (Fig. 2d). The GO term and STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) [77] analyses also ...
1b). This DNA substrate enabled us to determine, in an unbiased manner, which recessed end Rad24-RFC prefers to bind. We mixed purified proteins with the DNA and 0.5 mM ATPγS, a slowly hydrolyzable ATP analog. Cryo-EM analysis indicated assembly of the Rad24-RFC–9-1-1–DNA ...
4c). In contrast, KO of Lsm12 expression had no influence on the intracellular Ca2+ elevation induced by 1 µM extracellular ATP (Supplementary Fig. 4b), a purinergic receptor agonist that triggers IP3 production and Ca2+ mobilization from the ER. Thus, we conclude that Lsm12 is ...