We also apshsoaPye/PdlatchpheorPeQsisctoanmcpeleomf TenTt0ed1 to the synthetic Spodoptera frugiperda cecropin B (Sf-CecB1), to mimic the antimicrobial activities naturally found in the plasma of insect larvae challenged with bacteria30. A minor Sf-CecB1-resistant population of TT01 was also ...
Conclusions The data presented herein complement previous studies within the context of synthetic accessibility to EBN and efficient spin-trapping analysis of GS•. From all nitrones tested thus far, trans-Mito-DEPMPO, DEPMPO tae1xn/2pdEeBErNiB/mSNGe =nfot 1ar2ml0e sftfehocer)t....
X. laevis oocyte assays. Oocytes were micro-injected 1 h before incubation in 10 µM progesterone as pre- viously described96 (although with recombinant proteins rather than synthetic mRNA). GVBD was assessed by checking for the appearance of a white spot at the surface of activated...
Synthetic Aβ42 (GL Biochem, Shanghai, China) was dissolved in hexafluoroisopropanol (HFIP, Sigma, St. Louis, MO, USA). Furthermore, 100 µL Aβ was added to a clean tube and diluted with 900 µL Milli-Q water. HFIP in the solution was evaporated under N2 to until a final ...
Comparison of Hemolytic Activity of MP-V1 with the One of Other Mastoparans Hemolytic activity of the synthetic mastoparans was examined with 5 to 100 µM doses, as shown in Figure 2. Generally, there was notably little hemolysis in human erythrocytes treated with MP-L, -X(V) and -...
Also, 12 living Elysia viridis specimens were kept in glass aquaria, with artificial seawater (Instant Ocean®, synthetic sea salt, added according to instructions), salinity around 36–37.5 PSU, rela- tive density~1.0275–1.0286, at 17–19 ◦C, aerated via an oxygenation pump and air ...
(oIl)dc(oI)mcpolmexpelsexceosmcobminbeidnweditwh ittrhictlroiscalonsan Plasmonium falciparum and BioChPPellmaassP2LLmm0leea2iiPoo2ssLnn,mlhhae2iimmuusiosmnmmaahnniomuffiinaaaamaillnucciinnfiiimapapfflaaaacifnnrrnaiuupttlfuucaamminrmmputaaaumrnnmuddamndand Gold(IG) cooldm(Ip)lecxoemspcloexmesbic...
To create chimera DA, a synthetic gene with codons optimised for expression in E. coli of LC.HN/D domains was fused to the synthetic gene encoding HC/A. The resultant gene fusion encoding the protein chimera DA (LC.HN/D-HC/A) was subsequently cloned into a previously-reported construct,...
We have previously shown that a synthetic hit thioxanthonic molecule (1-{[2-(diethylamino)ethyl]amino}- 4-propoxy-9H-thioxanthen-9-one, TXA1) and its soluble salt (TXA1.HCl), reduced the in vitro growth of a panel of human tumor cell lines, without affecting the growth of non-...
Production and Purification of Recombinant NS1 To semi-purify secreted rNS1, Vero/ZIKV.NS1, cell monolayers were cultured for 4 days with synthetic Panserin 401 medium (PAN Biotech) that has been developed for serum-free cell cultivation. Non-denaturing concentration of rNS1 dimer in cell ...