A sequencing “library” must be created from the sample. The DNA (or cDNA) sample is processed into relatively short double-stranded fragments (100–800 bp). Depending on the specific application, DNA fragmentation can be performed in a variety of ways,...
The interaction between the LBD of LDLR and GETV particles was additionally confirmed using an enzyme-linked immunosorbent assay (ELISA). Here GETV virions were captured using a mouse anti-E1 monoclonal antibody (mAb). As demonstrated in Fig.3h, GST-LBD but not GST was able to interact with...
but the mechanisms contributing to IL-27’s suppressive effects are still poorly understood. Takahashiet al. identified the gene encoding cholesterol 25-hydroxylase (Ch25h), the enzyme that converts cholesterol to 25-hydroxycholesterol (25OHC), as strongly and ...
Plas- tidial thioredoxin z interacts with two fructokinase-like pro- teins in a thiol-dependent manner: evidence for an essen- 342 植物学报 49(3) 2014 tial role in chloroplast development in Arabidopsis and Nicotiana benthamiana. Plant Cell 22, 1498–1515. Aseeva E, Ossenbühl F, Sippel C...
To generate an enzyme inactive BcCrh1 protein, the catalytic residues were mutated (E120Q/D122H/E124Q) by site-directed PCR mutagenesis using template cDNA, and the amplification fragment was cloned into pJET plasmid to generate the pJET-MBcCrh1 vector. For generation of fungal strains that ...
Because a secreted amylase is isolated from plastidic starch, the function of this enzyme is difficult to predict, but circumstantial evidence suggests that it may degrade starch after cell death. The Arabidopsis thaliana genome contains three 伪 -amylase genes, one of which, AMY1 (At4g25000),...
In eukaryotes one and the same gene can be methylated both on cytosine and adenine residues; thus, there are, at least, two different and probably interdependent systems of DNA methylation in the cell. First higher eukaryotic adenine DNA-methyltransferase was isolated from plants; this enzyme ...
Increased expression of AhR mRNA has been noted in a human keratinocyte cell line (HaCaT cells) in response to arsenic[27]. In contrast, Cd has no effect on the AhR level in a human lung adenocarcinoma cell line (H1355 cells)[28]. An increase of AhR-regulated genes, including cytochrome...
Acting as an anti-oxidant, deuterium-enriched water prevents the toxic effect of such oxidative agents as hydrogen peroxide and auranofin. This action is opposite to that of deuterium depletion that is known to suppress cell growth and induce oxidative stress in mitochondria. We thus hypothesize ...
Because WT and RBDmut ERα are able to localize to the nucleus (Figure S2C), we next wanted to find out whether the ERα RBD mutant may have an effect on the DNA-binding function of ERα. To test this, we first extracted the chromatin-bound fractions of MCF7 cell lysates and ...