RNA was precipitated with ethanol in the presence of 2 mM EDTA and 2 M ammonium acetate. Biotinylated NAD-capped RNAs were captured by binding to 20 µL streptavidin magnet beads (Nvigen) at room temperature with gentle rocking for 1 h in 100 µL binding buffer (1 M ...
Mobile phase A consisted of 10% LC/MS grade acetonitrile (A955, Fisher), mobile phase B consisted of 90% LC/MS grade acetonitrile, and both mobile phases contained 10 mM ammonium acetate (73594, Sigma) and 0.2% acetic acid (A11350, Fisher). Column temperature was kept at 40 °C,...
Samples were neutralized by the addition of 3 M ammonium acetate, ethanol precipitated, and dissolved in H2O. The DNA fragment containing the STAT3 recognition sequence was amplified by PCR using the following set of primers: GFmS; 5′-GGGATTTATTAGGAGAATTTTAGTAAGTAG-3′, GFmAS; 5′-TCTACC...
tail with 20 lysines, and we speculate that replacement by the tailless Hζ-Hε variant might be facilitated by lysine acetylation, which would neutralize the basic charge of the Hβ-Hα C-terminal tail and detach it from its electrostatic contact with the acidic DNA wrapping around the ...
A lead-containing copper-based alloy is immersed into a weak acidic or neutral etching solution having a buffer effect which is formed by adding an organic acid into a complexing agent having a high ability to form a complexing ion with lead, and lead particles present on the surface of the...
G. Sewage sludge biochar influence upon rice (Oryza sativa L) yield, metal bioaccumulation and greenhouse gas emissions from acidic paddy soil. Environ. Sci. Technol. 47, 8624–8632 (2013). Article ADS CAS PubMed Google Scholar Wang, S. et al. Abundance and Functional Importance of ...
To confirm that PRDX6 inhibition leads to disruption of delivery of host cell cytosol to the FV, we preloaded RBCs with pH- dependent LysoSensor Blue/Yellow that emits blue fluores- cence at neutral pH, as found in the cytosol, and yellow...
The synthesis of the bis-isoxazolyl-1,2,5,6-tetrahydropyridine-3-carboxylates is achieved in high yield without the production oftoxic waste products by using room temperature ionic liquid (RTILs) triethylammonium acetate (TEAA). The RTIL TEAA played the dual role of efficient greensolvent as ...
X-ray crystallography. Crystals of hDdi2 RVP were grown by the hanging drop vapor diffusion technique at 19 °C with 0.2 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, and 25% PEG 3350 as precipitant. For cryoprotec- tion, crystals were soaked in the reservoir solution ...
For HILIC, the gradient went from 99% mobile phase A (0.1% formic acid, 10 mM ammonium acetate, 90% acetonitrile, 10% H2O) to 95% mobile phase B (0.1% formic acid, 10 mM ammonium acetate, 50% acetonitrile, 50% H2O). Both columns were run at 45 °C at a flow rate of ...