Skatole is a malodorous compound that contributes to the characteristic smell of animal faeces. Although skatole has long been known to originate from bacterial tryptophan fermentation, the enzyme catalysing its formation has so far remained elusive. Here we report the use of comparative genomics for...
Since the nitrosamines arerelatively polar organic compounds, a liquid-liquid extraction was employed for fat-rich food samples. Acetone-water (3 : 1) or acetone-acetonitrile mixture satisfied the terms. What do nitrites in urine mean? Normal urine contains chemicals called nitrates. If bacteria en...
Oxytetracy- cline concentrations were determined by Liquid Chromatography Mass Spectrometry (LCMS) on 100 ml fecal water mixed with 900 ml acetonitrile (containing 500 pg mlÀ1 of the internal standard demeclocycline hydrochloride). Quantitative real-time PCR The relative abundance of different ...
The reaction was performed at 30 °C for 0–20 min and terminated by the addition of 240 μl ice-cold acetonitrile to precipitate the proteins and extract MMC. The reaction mixtures were stored at −20 °C overnight and then centrifuged at 10,000 × g for 15 min ...
Both of these pathways would eventually contribute to a decreased bladder cancer risk. The present study may contribute to a better mechanistic understanding of environmental carcinogen-induced bladder cancer and provide a potential biomarker for predicting the susceptibility to human bladder cancer. ...
Cabozantinib metabolites were eluted isocratically by 5 mM ammonium acetate in water (pH 5) containing 60% acetonitrile solvent at a flow rate of 0.6 mL/min and measured by Q-ToF mass spectrometer (maXis Plus, Bruker Daltonics, Bremen, Germany) operating in positive data-dependent mode. Mass ...
a drift tube temperature of 30 °C, wavelength of 250 nm, using Xtimate C18 column (4.6 mm × 250 mm, 5 µm) at 30 °C for 50 min. The monosaccharides were eluted using 0.05 M potassium dihydrogen phosphate (PH 6.7)-acetonitrile mobile phase (83:17) at a flow rate of 1 mL/...
with a gradient of acetonitrile and water as the mobile phase. The detection was performed using a UV/Vis detector based on retention times. The measurement methods employed were rigorously validated to ensure data reliability. The measurements followed the N 12341:2023 standard [26], aligning with...
The compounds were separated using an isocratic elution with a reverse-phase C18 column (100 mm × 4.6 mm, Merck Chromolith Performance, Darmstadt, Germany) at 35 ◦C (7971 R Grace oven). The mobile phase consisted of a mixture with acetonitrile/methanol/water (10:30:60, v/v/v) with...