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Full size image To corroborate this finding, we employed an in vitro system using human pluripotent stem cell (hPSC) differentiation to recapitulate the earliest stages of haematopoietic development via EHT (Fig.4a). Briefly, we previously demonstrated that treating primitive streak-like cells with th...
Freeze substitution and embedding of the HPF-fixed samples were carried out in a temperature-controlled device, AFS2 (Leica Microsystems GmbH, Germany) at − 90 °C for 10 h, using 0.05% (w/v) Uranyl Acetate in dry acetone. The temperature was then raised to −45 °C (5 ...
aFolin–Trolox was from Aldrich. Acetone gradient grade for liquid chromatography 福-本-迈三氏法Trolox是从Aldrich。 丙酮梯度等级为液相色谱[translate] aAppendix 1: Specification 附录1 : 规格[translate] aIf you own 100% of the company, will you continue to support Gemtek Business? I want to he...
OBJECTIVE: The aim of this study was to determine the skeletal effects of Butea total extract (BTE) and its acetone soluble fraction (ASF) from Butea monos... R Pandey,AK Gautam,B Bhargavan,... - 《Menopause-the Journal of the North American Menopause Society》 被引量: 62发表: 2010年...
A process for preparing poly(urethane/urea) prepolymers and aqueous poly(urethane/urea) dispersions (PUDs) therefrom, the poly(urethane/urea) polymers having high heat resistance and good water resistance. The method employs a water soluble volatile organic solvent, such as acetone, at a level ...
The anti-inflammatory activity of curcumin and the synthetic analogs was tested in RAW264.7 cells stably expressing luciferase downstream of an NF-κB response element. The dose-dependent inhibition of LPS-induced luciferase activity by the analogs was expressed as IC50 values (Fig. 1A). Compounds ...
(albeit malformed) sacral region was appropriately spaced relative to the last formed thoracic element, defined by the presence of adjacent rudimentary pelvic/hindlimb bones (blue S, Fig.2B). Rostral to this, the transverse processes of all intervening post-thoracic vertebral elements assume a ...
The cells were then fixed with methanol/acetone (1:1) at −20 °C for 5 min with PBS, and stained with propidium iodide (PI; final concentration, 1 μg/mL) at room temperature for 10 min. The plates were imaged on an IncuCyte device (Essen Bioscience, Ann Arbor, MI, ...