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Behavior measurement We used video cameras (Canon, LEGRIA HF-806) to record the aggressive behavior of voles in both Experi- ment 1 and Experiment 2 from 10:00 to 12:00 a.m. at the last day of weeks 2, 4, 6, and 8 (Fig. 1c, d). By following Takahashi and Miczek (2014) ...
The primers used to amplify the region are: 319F: 5’-ACTCCTACGGGAGGCAGCAG-3’ and 806R: 5’-GGACTACHV GGGTWTCTAAT-3’. DNA sequencing was performed on the HiSeq 2500 platform (Illumina) using the HiSeq Rapid SBS Kit v2 Sequencing strategy [22]. Sequencing reactions and execution ...
Also, mice deficient in the innate immunity activator Toll-like receptor 2 show increased susceptibility to methotrexate-induced intestinal mucositis, an indication that microbial signaling could modify the severity of the response [11]. It is not completely clear how chemotherapy alters the oral ...