Hippocampal neurons obtained after centrifugation were seeded on poly-L-lysine (Sigma-Aldrich, P4707)-coated well plates. Cells were cultured in Neurobasal Medium (Thermo Fisher Scientific) supplemented with 1× B27 Supplement (50×; Thermo Fisher Scientific), 0.5 mM glutamine (Thermo Fisher ...
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COS-7 cells were cultured on poly-l-lysine (Sigma cat# P4707) coated 24-well tissue culture dishes in growth media 18–24 h priors to transfection. A transfection complex containing DMEM (Sigma cat# D5796), 1 μg of plasmid, and 2 μl of Lipofectamine 2000 (Invitrogen cat# ...
With the global population aging, the number of individuals over 60 is expected to double by 2050. Brain volume increases until age 13, stabilizes between
Poly-L-Lysine Solution Sigma-Aldrich P4707-50ML Wortmannin Sigma-Aldrich W1928 Adenosine 5′-diphosphate sodium salt Sigma-Aldrich A2754-100MG U-73122 Sigma-Aldrich U6756 Thrombin Sigma-Aldrich T4648-1KU Annexin V-FITC Merk APOAF-50TST D-(+)-Glucose Sigma-Aldrich G7021 Sucrose Samchun Chemic...
1SAR111050R4707 A06B-6110-H011 2090-CTPW-MADF-18A03 2080-L50E-24QBB DSDI120 DSDO131 1606-XLP90E-2 FSPS-21 3UF5041-3AN00-1 FCNA-01 6L3244-0BB12-1BA1 6SL3210-1PE18-0UL1 20BC105A0AYNANC0 ATV312HU40N4 4KW 6ES7288-2DE16-0AA0 ...
Poly-L-Lysine (PLL) Sigma Cat#P4707-50ml Papain Worthington Cat#LS003126-100 g Fluoromount-G Thermo Fisher Scientific Cat#0100-01 Superfect QIAGEN Cat#301307 Fastgreen Sigma Cat#F7258-25 g TRIzol GIBCO Cat#15596018 Sorbitol Sigma Cat#S8143-1kg Na-pyruvate Sigma Cat#P-5280 Protease Inhibito...
In part, this is due to the absence of an appropriate biochemical system that functionally recapitulates RNA pol II pausing and elongation and with which the pausing machinery can be identified. We describe herein a cell-free system (CFS) derived from HeLa cells that recapitulates pausing and ...
Proc Natl Acad Sci USA 1990; 87: 4707–4711. Article CAS PubMed PubMed Central Google Scholar Licht CL, Stevnsner T, Bohr VA . Cockayne syndrome group B cellular and biochemical functions. Am J Hum Genet 2003; 73: 1217–1239. Article CAS PubMed PubMed Central Google Scholar Laine ...
e44707 View in ScopusGoogle Scholar 44 F. Sonntag, S. Bleker, B. Leuchs, R. Fischer, J.A. Kleinschmidt Adeno-associated virus type 2 capsids with externalized VP1/VP2 trafficking domains are generated prior to passage through the cytoplasm and are maintained until uncoating occurs in the ...