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1g, h; Supplementary Fig. 1m). These results suggest that p62, a well characterized autophagic adapter, binds type-1 and type-2 destabilizing N-terminal residues and may function as an N-recognin. p62 ZZ domain is equivalent to the UBR box of N-recognins UBR1 recognizes type-1 and ...
Briefly, detached plant leaves were soaked overnight in one of the following staining solutions: lactophenol cotton blue (0.5 mg/ml of cotton blue dissolved in lactophenol containing an equal volume of lactic acid, glycerol, liquid phenol and ddH2O), trypan blue (2.5 mg/ml of trypan ...
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Here, your input should be equal to 3d3d516343746d4d6d6c315669563362, but it is modified by the encodeSecret() function.We just need to reverse it to obtain the right secret. Here is the Python script :import base64 secret = "3d3d516343746d4d6d6c315669563362" secret = bytes.fromhex(secret...
PLA is equal to TLA minus chlorotic areas. • Cold treatments Seeds were stratified as described earlier (Monitored stress applied in propagation plugs). Then a large-scale screen to test cold tolerance was performed as follows: rows of plants were sown in square pots containing organic ...
The binding competition of eIF3j and Nsp1 to the 40S was tested at different concentrations. There is little eIF3j binding to the 40S when the concentration of eIF3j is equal or lower than that of Nsp1, and residual eIF3j binding was observed only when its concentration is higher than ...
Figure 1. Protein-Targeting Activity Orchestrated by the Native Long-Hairpin Fold of SRP RNA (A) Left to right: the signal recognition particle (SRP) inE. coliis composed of SRP RNA (red) and an SRP protein, Ffh (blue), whose M-domain binds to RNA hairpin-tip proximal stem. Thisribonu...
DNA:DNA:RNA triplexes that are formed through Hoogsteen base-pairing of the RNA in the major groove of the DNA duplex have been observed in vitro, but the extent to which these interactions occur in cells and how they impact cellular functions remains elusive. Using a combination of bioinforma...
B cells, deletions and inversions were present at nearly equal frequencies (57 vs 43%) (Fig. 5i). This normalization of the orientation ratios correlated with the level of DNA end resection in these two B cell types (Fig. 5h), suggesting that, in the absence of SHLD1, exacerbated resect...