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Whole-genome sequencing was performed by GeneWiz (South Plainfield, NJ) using contiguous amplicon sequencing on an Illumina MiSeq instrument in a 2 × 150-bp paired-end configuration. DNA library preparation without fragmentation, multiplexing, sequencing, unique sequence identification, and relative...
The normal rat kidney (NRK) cell line was cultured in Dulbecco’s modified Eagle medium ((DMEM) (Gibco, 41965-039)) and 10% fetal bovine serum (FBS). The human kidney (HK-2) cell line was cultured in Keratinocyte-SFM medium (Gibco, 17005-075). For luciferase assays, NRK cells were...
(<150 ×109and ≥1.5 × upper limit of normal, respectively) based on results from local laboratories, but the serum creatinine criteria (≥upper limit of normal) must have been confirmed by the central laboratory at baseline. Postpartum orrenal transplantpatients were permitted but must have ...
- Temp Grades: Extended: -40°C to +105°C Auto Grade (A3): -40°C to +125°C High Performance Serial Flash (SPI) - 50MHz Normal and 133Mhz Fast Read - 532 MHz equivalent QPI - DTR (Dual Transfer Rate) up to 66MHz - Selectable Dummy Cycles - Configurable Drive Strength -...
The Persistent Protection method allows boot code the option of changing sector protection by programming or erasing the PPB, then protecting the PPB from further change for the remainder of normal system operation by clearing the PPB Lock bit. This is sometimes called Boot-code controlled sector ...
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This can be explained by the fact that most SM BGCs are silent under normal laboratory conditions. SM BGCs are controlled by a complicated gene regulatory network, and the understanding of gene regulation of fungal secondary metabolism is still limited16. Compared to other types of regulation, ...