TBK1. Cells were further treated with EGCG (20, 50 μM) or catechin (20, 50 μM) for 6 h. Relative luciferase activity (RLA) was determined and expressed as mean ± SEM (n = 3).∗∗, Significantly different from TBK1 plus vehicle, p < 0.01. (E)In vitroTBK1 kinase assay was...
It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear ...
The cGAS/STING/TBK1/IRF3 innate immunity pathway maintains chromosomal stability through regulation of p21 levels Abdul Basit, Min-Guk Cho, Eui-Yun Kim, Dohyeong Kwon, Suk-Jo Kang & Jae-Ho Lee Experimental & Molecular Medicine volume 52, pages 643–657 (2020)Cite this article 30k Accesses...
STINGSpecifiesIRF3phosphorylationbyTBK1intheCytosolic DNASignalingPathway YasuoTanaka1andZhijianJ.Chen1,2,* 1DepartmentofMolecularBiology,UniversityofTexasSouthwesternMedicalCenter,Dallas,TX 75390-9148,USA 2HowardHughesMedicalInstitute,UniversityofTexasSouthwesternMedicalCenter,Dallas,TX 75390-9148,USA Abstract Cytos...
(Zhang and Pagano, 1997). IRF7, once induced, is present in the cytoplasm as an inactive protein that is also phosphorylated on specific C-terminal residues by TBK1 and/or possibly IKKε kinases (Fitzgerald et al., 2003;Sharma et al., 2003). IRF7 has an important role in the ...
multiplicity of infection; PAMPs: pathogen-associated molecular patterns; SeV: Sendai virus; siRNA: small interfering RNA; SQSTM1/p62: sequestosome 1; STING1: stimulator of interferon response cGAMP interactor 1; TBK1: TANK binding kinase 1; Ub: ubiquitin; WT: wild-type; VSV: vesicular stomatit...
The transcription factor inter- feron regulatory factor 3 (IRF3) is then recruited to the signaling complex and phosphorylated by TBK111,15. Phosphorylated IRF3 initiates the expression of IFN-β , which regulates the expression of over two hundred interferon-inducible genes that can down regulate...
Activation of the transcription factor IRF3 is a key event in antiviral responses. Lichty and colleagues show that recruitment of the mTOR downstream effector S6K1 to the STING-TBK1 signaling complex is required for the activation of IRF3 after infection
(TLR) signaling,is phosphorylated by IKBKE and TBK1 kinases.This induces a conformational change,leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1),a complex which activates the transcription of the type I...
Upon virus infection, AXIN1 undergoes a phase separation triggered by phosphorylated TANK-binding kinase 1 (TBK1). This leads to increased phosphorylation of IRF3 and a boost in IFN-I production. Moreover, KYA1797K, a small molecule that binds to the AXIN1 RGS domain, enhances the AXIN1-...