心肌细胞（CMs）是心脏的肌肉细胞，通过跳动产生机械张力来维持心脏功能。CMs的机械输出来源于沿肌纤维排列的肌节的缩短。人iPS可以分化为跳动的CMs（hiPSC-CMs），但hiPSC-CMs中肌原纤维紊乱，不像原代CMs中那样排列整齐，这种混乱一直是应用hiPSC-CMs进行体外心功能分析的限制因素。基质微图案化培养hiPSC-CMs可以成...
iPSC-CM是探索抗精神病药物致心脏毒性作用机制的宝贵工具。以iPSC-CMs为模型,通过MEA进行电信号分析,可以评估测试化合物对场电位(FP)的影响以及iPSC-CM中心律失常样波形的发生。实验采集的数据为iPSC-CM群落整体的场电位变化,而不是单纯的某一个离子通道的变化正。根据检测到的场电位时程FPD(Field Potential ...
图3:细胞功能成像显示HCM-iPSC-CMs舒张功能障碍。同时记录心肌细胞钙瞬变和细胞收缩应力,与Ctrl-iPSC-CMs相比,HCM-iPSC-CMs的钙瞬变幅值未发生显著变化,但是钙瞬变速率和肌丝钙敏感性均增强。 图4:β-肾上腺素处理加重HCM-iPSC-CMs舒张功能障碍。ISO处理进一步提高了HCM-iPSC-CMs中舒张期细胞内Ca2+浓度,显著延长...
Patient-specific induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) inherit the genetic background of the patient and reflect the electrophysiological characteristics of the native cardiomyocytes. Thus, iPSC-CMs provide an innovative and translational pivotal platform in car...
不同药物对 iPSC-CMs 细胞电生理信号影响斯高首页 ꄲ 不同药物对 iPSC-CMs 细胞电生理信号影响 创建时间:2024-04-19 18:00 넶浏览量:18 扫一扫 关注我们 版权所有 © 斯高电生理研究院 地址: 河南省开封市宋城路92号科创中心斯高研究院 电话: 400-059-7008 邮箱: infor@epscopelab.com 走进...
hiPSC-CMs潜在应用 局限性 体外产生的hiPSC-CMs可能无法完全概括心脏的复合物体内环境,并且可能无法准确反映心脏中发现的心肌细胞亚型的多样性。此外,hiPSC-CMs可能不适用于所有类型的心血管研究,例如需要完整心脏组织或整个动物模型的研究。这时候心脏工程组织更具优势。此外,hiPSC-CMs的幼稚性问题一直是存在的研究焦点。
Transcriptomics, proteomics, metabolomics and network pharmacology reveal molecular mechanisms of multi-targets effects of Shenxianshengmai improving human iPSC-CMs beating 【前言】 心动过缓是常见的心血管疾病之一;然而,临床上有效的口服药物并不多见。参仙生脉口服液是国家药品监督管理局批准的中药之一,本研究...
What effects does Blebbistatin have on the action potential and field potential of Induced Pluripotent Stem Cell Derived Cardiomyocytes (iPSC-CMs)? How was the Automated Patch Clamp used to study disease modeling in Induced Pluripotent Stem Cell Derived Cardiomyocytes (iPSC-CMs) under BB-buffered cond...
Furthermore, the in silico investigation yielded mechanistic insights; e.g., through simulations, bepridil’s more proarrhythmic action in adult cardiomyocytes compared to hiPSC-CMs could be traced to the different expression of ion currents in the two. Therefore, our work 1) supports the utility...
A novel ionic model for matured and paced atrial-like human iPSC-CMs integrating I K u r IKur and I K C a IKCa currents This work introduces the first atrial-specific in-silico human induced pluripotent stem cells-derived cardiomyocytes (hiPSC-CMs) model, based on a set of phenotype-...