"Transplantation of human embryonic stem cells onto a partially wounded human cornea in vitro." Acta ophthalmologica 91.2 (2013): 127-130. 3. Norrman, Karin, et al. "Distinct gene expression signatures in human embryonic stem cells differentiated towards definitive endoderm at single-cell level....
Robust growth of human induced pluripotent stem (iPS) cells in the Cellartis DEF-CS Culture System. The number of iPS cells was quantified after being cultured for three weeks using either the Cellartis DEF-CS Culture System, a reference feeder system, or four other stem cell culture systems. ...
) occurs in cells of indigenous T. piniperda from several European localities, as well as in insects introduced in North America (Kohlmayr et al., 2003). This pathogen can infest the midgut epithelium, the fat body, and the gonads. Chytridiopsis typographi (Weiser) is a non-specific ...
In vitro induction of mouse men⁃ ingeal-derived iPS cells into neural-like cells[J]. Chin Sci Bull, 2011,56(15):1556-1561.Xu J,Wang H,Liang T. In vitro induction of mouse meningeal-derived iPS cells into neural-like cells[J].Cell Biology,2011,(15):1556-1561....
1.1 Biology Ips is one of the better-known bark beetle genera because of several species that are destructive to conifer forests and plantations (Chararas, 1962; Furniss and Carolin, 1992). Species utilize Pinus, Picea, and uncommonly Larix, Abies, and Cedrus as a food resource for larvae ...
Another level of utility not yet addressed by TissUse but surely on the horizon is patient-specific testing of medications outside the body usingiPS cells(inducible pluripotent stem cells). Scientists create these cells from human skin or other tissues and “reprogram” them to become cells of ...
Methods for reprogramming human cells are unable to prospectively distinguish bona fide induced pluripotent stem (iPS) cells from partially reprogrammed cells. Using live imaging to monitor cell fate, Chan et al. identify a set of markers that allows ide
Notably, reprogrammable mice present circulating iPS cells in the blood and, at the transcriptome level, these in vivo generated iPS cells are closer to embryonic stem cells (ES cells) than standard in vitro generated iPS cells. Moreover, in vivo iPS cells efficiently contribute to the ...
We treated cells with varying concentrations of CHIR and noggin alone and in combination and then applied CellROX green fluorogenic indicator to detect the level of cellular ROS. The dye becomes fluorescent and binds to DNA after being oxidized by O2− and/or •OH in the living cells. Data...
In this chapter, the focus is turned from the hypothetical and anticipatory perspective of stem cell-based clinical translation to the “realities” of laboratory life with human pluripotent stem cells. Here practical challenges of instrumentalizing the artificially created iPS cell lines and their vola...