iProof ™ High-Fidelity DNA PolymeraseAmplification, RelatedFrom, ProductsContents, Kit
伯乐Bio-Rad1725301iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl,产品订购信息: iProof High Fidelity DNA Polymerase, Master Mixes, and Buffers 伯乐Bio-Rad 1725300 iProof High-Fidelity DNA Polymerase, 2 U/ul, 20 U, includes 5x reaction buffers, MgCl solution, DMSO ...
High fidelity— iProof DNA polymerase is 52-fold more accurate thanTaqpolymerase Speed— high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times Successful amplification of long products with higher yields— fragments up to 37 kb are amplified in less time and wi...
Figure 1. Proofreading by DNA polymerase corrects errors during replication. Some errors are not corrected during replication, but are instead corrected after replication is completed; this type of repair is known asmismatch repair(Figure 2). The enzymes recognize the incorrectly added nucleotide and ...
DNA polymerase I only contained a double-strand dependent 5′ → 3′ exonuclease activity but lacked any detectable 3′ → 5′ proofreading exonuclease activity. The lack of 3′ → 5′ exonuclease function in a variety of thermostable repair DNA polymerases may reflect enhancement of thermostabilit...
The θ Subunit of Escherichia coli DNA Polymerase III: a Role in Stabilizing the ∊ Proofreading Subunit. The function of the θ subunit of Escherichia coli DNA polymerase III holoenzyme is not well established, θ is a tightly bound component of the DNA polyme... SA Taft-Benz,RM Schaap...
Escherichia coli DNA polymerase I exists in at least two distinct kinetic forms. When it binds to a template, the proofreading activity is usually switched off. As the enzyme progresses along the template, it becomes more and more competent for excision. This phenomenon introduces a link between...
Keywords: Amino Acid Sequence,Animals,Bacteria,enzymology,Bacterial Proteins,chemistry,metabolism,Caenorhabditis elegans,DNA Polymerase I,DNA, Bacterial,Drosophila melanogaster,Escherichia coli,Exonucleases,Fungal Proteins,Helminth Proteins,Insect Proteins,Markov Chains,Mice,Models, Molecular,Molecular Sequence Data...
The incorporated Ara-CMP is efficiently removed by the proofreading exonuclease activity of polymerase epsilon (Polε), in which the alternative clamp loader CTF18 plays a pivotal role. However, the requirement of CTF18 for the removal of the other arabinosides from the 3′ end of nascent DNA...
PCR performance of the highly thermostable proof- reading B-type DNA polymerase from Pyrococcus abyssi. FEMS Microbiol. Lett. 217:89-94.Dietrich J, Schmitt P, Zeiger M, Preve B, Rolland JL, Chaabihi H, Gueguen Y (2002) PCR performance of the highly thermostable proof-reading B-type DNA ...