Herein, we encapsulated the mRNA expressing Uox (mUox) in ionizable LNP, composed of thermostable ionizable lipid A1-D1-5 (N1,N4-bis(3-(bis(2-hydroxydodecyl)amino)propyl)succinamide)25,26, DOPE (1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine), CHO (cholesterol), and DMG-PEG2000 (1,2...
Given the ability of ionizable lipids to enhance endosomal escape in a context of lipid nanoparticles (LNP), here, we provide the first report on the effect of an ionizable lipid conjugate on siRNA endosomal escape, tissue distribution, efficacy, and toxicity in vivo. After developing a ...
For ionizable poorly soluble drugs, a solvent-assisted active loading technology was developed to use miscible solvent assist drugs to go cross the lipid membrane, which was easier than the preceding way20. This facile approach was powerful in sweeping away the obstacle of poorly soluble drugs; ...
Meanwhile, ionizable lipid termed A1-D1-5, together with DOPE, cholesterol and PEG-DMG2000, were used to load mRNA (Fig. 1A and Supplementary Fig. S1), and the molar per- centage among four lipids was 35.1%: 11.7%: 52.6%: 0.6%. The mass percentage among four lipids was 52.6%: ...