Ion exchange chromatography serves as the workhorse in downstream processing and plays a significant role in impurity removal. Our large portfolio of anion and cation exchange resins are applied in many commercial and late-stage processes for the manufacturing of: ...
ionexchangechromatography 一、原理 离子互换层析是用离子互换剂（具有离子 互换性能旳物质）作固定相，利用它与流动相中旳离子能进行可逆旳互换性质来分离离子型化合物旳层析措施。➢即溶液中旳离子同离子互换剂上功能基团互换反应旳过程。带电荷量少，亲和力小旳先被 洗脱下来，带电荷量多，亲和力大旳后被洗脱下来...
ion exchange chromatography (IEC) in common use for more than 50 years, for separation and purification of proteinsstationary phase—the ion exchangers matrix with acidic or basic groups on attached ligand, basic ion exchangers or anion exchangers with positive groups and cation exchangers...
离子交换色谱(Ion Exchange Chromatography,简称IEC)是一种常用的蛋白质分离和纯化技术。它利用固定在色谱柱上的离子交换基团与蛋白质中的带电氨基酸残基之间的相互作用来实现分离。分辨率是衡量色谱技术分离效果的重要指标,它反映了样品中不同组分之间的分离程度。
离子交换层析(Ion Exchange Chromatography 简称为IEC)是以离子交换剂为固定相,依据流动相中的组分离子与交换剂上的平衡离子进行可逆交换时的结合力大小的差别而进行分离的一种层析方法。 1848年,Thompson 等人在研究土壤碱性物质交换过程中发现离子交换现象。本世纪40年代,出现了具有稳定交换特性的聚苯乙烯离子交换树脂。
摘要: Summary This chapter contains sections titled: Introduction Principle Properties of ion exchangers Influence of the mobile phase Special possibilities of ion exchange Practical hints Applications关键词: ion-exchange chromatography ion-moderated partition chromatography Ligand-exchange reactions calcium-...
Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography, in which positively charged ions bind to a negatively charged ...
Ion-exchangechromatographyisatech- niquewithveryhighbindingcapacities, highflowcharacteristicsandpotentially excellentresolution.Itisthereforeperfect fortheseparationoflargevolumesof sample(fluidfeed)andfitswellinto theearlyorcapturestepofapurification methodology. ...
This also underlies the different pIs of proteins (see Chapter 23). Ion-exchange chromatography (1) separates proteins first on the basis of their charge type (cationic or anionic) and, second, on the basis of relative charge strength (e.g., strongly anionic from weakly anionic)....
Ion-exchange chromatography (often referred to as ion chromatography) allows the separation of ionic molecules on the basis of their charge. From: Encyclopedia of Forensic Sciences, Third Edition, 2023 About this pageAdd to MendeleySet alert ...