Simplified workflow—Buffer formulated for primer annealing at 60°C (no need for a Tm calculator) Increased PCR success—Robust amplification of GC-rich targets, DNA of suboptimal purity, and long sequences Improved automation—High specificity and benchtop stability for 24 hours after reaction setup...
70°C. Annealing temperatures are generally about 5°C below the Tm of the primers. Since most formulas provide an estimated Tm value, the annealing temperature is only a starting point. Specificity for PCR can be increased by analyzing several...
Tm Calculator DNA Ladders Selection Guide PCR Enzymes PCR and qPCR Plastic Consumables Reverse Transcriptase Restriction Enzyme When the polymerase chain reaction (PCR) was first introduced, the process was challenging and time con...
70°C. Annealing temperatures are generally about 5°C below the Tm of the primers. Since most formulas provide an estimated Tm value, the annealing temperature is only a starting point. Specificity for PCR can be increased by analy...
70°C. Annealing temperatures are generally about 5°C below the Tm of the primers. Since most formulas provide an estimated Tm value, the annealing temperature is only a starting point. Specificity for PCR can be increased by analy...
Tm Calculator DNA Ladders Selection Guide PCR Enzymes PCR and qPCR Plastic Consumables Reverse Transcriptase Restriction Enzyme When the polymerase chain reaction (PCR) was first introduced, the process was challenging and tim...
Tm Calculator DNA Ladders Selection Guide PCR Enzymes PCR and qPCR Plastic Consumables Reverse Transcriptase Restriction Enzyme When the polymerase chain reaction (PCR) was first introduced, the process was challenging and time cons...
Tm Calculator DNA Ladders Selection Guide PCR Enzymes PCR and qPCR Plastic Consumables Reverse Transcriptase Restriction Enzyme When the polymerase chain reaction (PCR) was first introduced, the process was challenging and time con...
Tm Calculator DNA Ladders Selection Guide PCR Enzymes PCR and qPCR Plastic Consumables Reverse Transcriptase Restriction Enzyme When the polymerase chain reaction (PCR) was first introduced, the process was challenging and time cons...
Tm Calculator DNA Ladders Selection Guide PCR Enzymes PCR and qPCR Plastic Consumables Reverse Transcriptase Restriction Enzyme When the polymerase chain reaction (PCR) was first introduced, the process was challenging and time con...