resistance genes for your choice of selection in E. coli ? T7 promoter/priming site for in vitro RNA transcription and sequencing ? M13 forward and reverse primer sites for sequencing Novel Protocol The TOPO? XL PCR Cloning Kit protocol includes S.N.A.P.? gel purification which employs ...
•Simple—a single digestion protocol for all DNA types •Complete system—incorporates Anza DNA Modifying Enzymes for a complete cloning experience Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza Alkaline Phosphatase Kit, Anza T4 PNK Kit, Anza DNA Blunt End Kit, an...
is supplied for added convenience. Digestion reactions are as efficient in the Anza Red Buffer as in the Anza Buffer. Use of the Anza Red Buffer helps reduce pipetting steps when preparing multiple digestion reactions and a subsequent step includes agarose gel electrophoresis. For applications that ...
Plant RNA Reagent is optimized to have less sample DNA contamination, to be twice as effective as TRIzol® Reagent, and have a simplified RNA extraction protocol for minimized time and labor. The end result is high-yield, high-purity RNA (determined by A260/A280and gel analysis) from a ...
Trizol® is an excellent reagent. The protocol is easy to follow and allows interruption after the homogenization when samples can be frozen at -80°C, if necessary. Invitrogen's website contains the well-explained protocol with hints for working with Trizol® and RNA and FAQs about the me...
WASH your IPS or pulldowns prior to running them on the gel. Your bait or antibody will be at a high concentration while what you are looking for will be relatively weak. When you start the developing portion of the protocol you will immediately see your bait or antibody and it will tak...
staining. The background was higher in lower percentage (5%) gels as compared to higher percentage gels (10%, 12% gels) due to increased trapping of the Colloidal dye in the bigger pores. Longer destaining procedures are then necessary to completely destain the gel for quantification of the...
The protocol should be followed precisely, however; skipping the 3 water rinses before staining has resulted in a complete lack of staining in my lab. These washes are necessary. However, I have left the gel for longer than 5 minutes for each of the rinses and this has had no deleterious...
S21900SYPRO RUBY PROTEIN GEL5 L9884 S22801SNARF-1 CARBOXYLIC ACI10 X 50 UG3237.5 S23371STAPHYLOCOCCUS AUREUS (WO2 MG1624 S23372STAPHYLOCOCCUS AUREUS (WO2 MG1624 S23753SPHINGOSTRIPS(TM) MEMBRAN1 SET4746 S23920SNARF(R)-4F 5-(AND-6)-CAR1 MG2660 S23921SNARF-4F 5-(AND-6)-CAR20 X 50...
A protocol was developed to determine the ability of amino acid denaturants to denature or separate double-stranded nucleic acid molecules to form single-stranded nucleic acid molecules (e.g., double-stranded DNA to form single-stranded DNA molecules). In this method, pSPORT I-CAT DNA is us...