化学感受态细胞 (One Shot? Stbl3? Chemically Competent Cell) 提供: 更高的基因组克隆容量 (mcrB, mrr) >1 x 108 转化子?μg 的效率 非常高的 DNA 产量 基因型:F- mcrB mrr hsdS20 (rB-, mB- ) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(Str ) xyl-5 λ- leu mtl-1r Invitrogen...
Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 108 cfu/µg plasmid DNA. Library Efficiency DH5α Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amoun...
MAX Efficiency DH5αF´IQ Chemically Competent Cells are high-efficiency chemically competent cells available for M13 cloning, cloning into plasmids with an f1-like origin of replication (phagemids), or cloning into any vector that uses expression from the lac promoter.MAX Efficiency DH5αF´...
One Shot® TOP10 Chemically CompetentE. coliare provided at a transformation efficiency of 1 x 109cfu/µg plasmid DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids and are the same competent cells that come with...
The TA Cloning® Kit is offered in a variety of configurations: with One Shot® INVF' Chemically Competent E. coli (K2050-01 and K2050-40), One Shot® TOP10F' Chemically Competent E. coli (K2060-01 and K2060-40), and without competent cells (K2750-20 and K2750-40) in 20...
The ligated mixture may be used directly in transformation of chemically competent cells but may require purification prior to transformation of electrocompetent cells. If PEG was used in the ligation reaction, heat inactivation of the ligase is...
coli Wet icery ice –20°C80°CLenti6/V5-DEST Gateway®Vector Kit: Vectors One Shot®Stbl3™Chemically Competent E. coli Wet icery ice –20°C80°CLenti6.2/V5-DEST Gateway®Vector Kit: Vectors One Shot®Stbl3™Chemically Competent E. coli Wet icery ice –20°C80°CLenti6.2/C...
The ligated mixture may be used directly in transformation of chemically competent cells but may require purification prior to transformation of electrocompetent cells. If PEG was used in the ligation reaction, heat inactivation of the ligase is...
Any recipient cell may be used, including prokaryotic or eukaryotic cells, although prokaryotic cells and bacteria, such as gram negative bacteria, are preferred. Particularly preferred gram negative bacteria include E. coli, Salmonella, Klebsellia, etc. Electrocompetent and chemically competent E. coli...
PCR samples were then transformed into 100 microliters of MAX Efficiency DH10B™ Competent Cells (Life Technologies, Inc.; Rockville, Md.). The mutated portion of the GFP cDNA was then subcloned with a NotI and BamHI digest into the pGreenLantern-2 backbone (FIG. 6) which was not ...