This invention relates to synthetic adeno-associated virus (AAV) inverted terminal repeats (ITRs) that exhibit altered activities compared to a naturally occurring AAV ITR and methods of using the same for delivery of nucleic acids to a cell or a subject. The synthetic ITRs provide a larger ...
The inverted terminal repeats (ITRs) of the MPOX are made up of hairpin loops, tandem repeats, and a few open reading frames (ORFs), and the MPOX is a 197 kb linear ds-DNA covalently joined at its ends by palindromic hairpins [40]. Variable ends with inverted terminal repeat protect ...
Two copies of a new mariner -like element ( MLE ) presenting unusual inverted terminal repeats (ITRs), Mcmar1-1 and Mcmar1-2 , were cloned and sequenced in the genome of the phytoparasitic nematode Meloidogyne chitwoodi . Although the sequence features of these Mcmar1 transposons are commonp...
Inverted terminal repeats (ITRs) of the adeno-associated virus (AAV) are essential for rescue, replication, packaging, and integration of the viral genome. While ITR mutations have been identified in previous reports, we designed a new truncated ITR lacking the B-B’ and C-C’ regions named ...
3.The synthesis and cloning of Adeno-as-sociated virual inverted terminal repeats腺病毒反向重复序列ITRs的人工合成与克隆 4.Genetic Transformation of Inverted Repeat Chimeric Gene from CMV and TuMV;CMV和TuMV病毒嵌合基因反向重复表达载体的构建及其遗传转化 5.Virus Resistance Mediated by Inverted Repeats Der...
is 4,675 nucleotides in length and contains inverted terminal repeats of 145 nucleotides, the first 125 nucleotides of which form a palindromic sequence. ... A Srivastava,EW Lusby,KI Berns - 《Journal of Virology》 被引量: 824发表: 1983年 Comparative sequence analysis of the inverted terminal...
This invention relates to synthetic adeno-associated virus (AAV) inverted terminal repeats (ITRs) that exhibit altered activities compared to a naturally occurring AAV ITR and methods of using the same for delivery of nucleic acids to a cell or a subject. The synthetic ITRs provide a larger ...
Replication of the palindromic inverted terminal repeats (ITRs) of adeno-associated virus type 2 requires several functions of the viral nonstructural Rep proteins. These include binding to the ITR, nicking of the double-stranded replication intermediate at the terminal resolution site (trs), and then...
In some aspects, the disclosure provides recombinant AAV and nucleic acid constructs having novel inverted terminal repeats (ITRs), cap, and/or rep genes. In some aspects, the disclosure relates to gene transfer methods using rAAVs described herein....
One of the primary challenges in working with adeno-associated virus (AAV) lies in the inherent instability of its inverted terminal repeats (ITRs), which play vital roles in AAV replication, encapsidation, and genome integration. ITRs contain a high GC content an...