Figure 2. Morphology of migrating B16F10 cells from the time-lapse microscopy of the wound closure assay. During a 16-hour wound closure assay pictures were taken every 5 minutes. All pictures were assembled into a video using the ImageJ program (5 frames/second). Cell migration involving cel...
Cell migration and invasion assay Cells were planted in a 6-well plate at a density of 8 × 105 cells for each well. After the cells reached confluence, a single scratch was made across the cell monolayer using a pipette tip. Subsequently, the cells were cultured in a medium without...
Here, we present an optimized ex vivo brain slice invasion assay that models glioblastoma multiforme (GBM) cell invasion into organotypic brain slices. Using this model, human GBM spheroids can be implanted with precision onto murine brain slices and cultured ex vivo to allow tumour cell invasion ...
C Viral titers, as measured by plaque assay, from olfactory bulb, cortex, and sera collected at various DPI from mice co-administered intranasal IFNα during ZPC-738 (10 pfu) infection (N = 6). D Immunostaining of intranasal VEEV–ZPC–GFP infection (50 pfu, 3dpi) of sagittal ...
TUNEL assay The cells were washed twice with PBS, fixed in 4% paraformaldehyde and stained; after which, they were visualized using a one-step TUNEL kit (C1089, Beyotime Institute) as previously described [19]. Fluorescence density was analyzed using Image Pro plus 6.0 software. Sections of ...
In addition, in transwell cell invasion assay, miR-183-S1 cells showed a significant increase of 2.56 folds and miR-492-S1 cells of 2 folds in the number of Matrigel-invading cells compared to control S1 cells. miR-Control-S1 cells did not show any significant increase in invading cells ...
A scratch assay was utilised to assess cell migration. Cells were seeded in 6-well plates and allowed to reach confluence. A sterile 200-µL pipette tip was used to create a straight scratch across the cell monolayer. After washing with phosphate-buffered saline (PBS) to remove detached cel...
D. & Massague, J. Metastasis: from dissemination to organ-specific colonization. Nat. Rev. Cancer 9, 274–284 (2009). CAS PubMed Google Scholar Albini, A. et al. A rapid in vitro assay for quantitating the invasive potential of tumor cells. Cancer Res. 47, 3239–3245 (1987). CAS...
J-K: mRNA stability assay measuring APC and GSK3β mRNA half-life in YTHDF2 or PRMT6 overexpressed cells Full size image The transcriptional activation of YTHDF2 by PRMT6 requires its methyltransferase activity, and inhibiting this enzymatic activity can suppress the malignant phenotype of glioma...
Quantitation of live-imaging experiments was performed using Image J software. Invasion assay. Merozoites were purified as previously described (31). Briefly, highly synchronized ring-stage PfPKAc:loxP parasites were treated with DMSO or rapamycin and grown until they became late-stage schizonts. ...