The introduction of purified DNA into bacteria is an almost universally applicable means of transferring genes between organisms, and has the major advantage that no vector is required: the nucleic acid itself mediates information transfer. The term transformation was originally applied to gene transfer...
‘selected bacteria’ cultures and complex ecological systemDNA probe donor strainDNA probe constructionnon‐radioactive hybridizationBluGENE™ reaction kit and Gibco BRLstreptococci of ‘selected bacterial culture’dot blot hybridizationMaterials and Methods Results and DiscussionConclusions Acknowledgements...
However, natural bacterial transformation occurs at a low frequency, 10-2–10-10, and highly variable between bacteria species [1,2]. With the advent of molecular cloning in the 1970s, the process of transformation was leveraged to ...
The second section of the book describes DNA repair and genome instability in prokaryotes. This section includes chapters by Ashley Williams and colleagues in which they compare genome stability of bacteria and archaea (Chapter 4), and show the role of the environment in bacterial mutagenesis (Chapt...
1The introduction of mass-production methods enabled many people ___ and gave them an unprecedented amount of mobility. A. to purchase their own automobiles B. their own to purchase automobiles C. to their own purchase automobiles D. own their automobiles to purchase 2The introduction ...
Traditional methods of cloning a DNA sequence into a vector and replicating it in a living cell often require days or weeks of work, but amplification of DNA sequences by PCR requires only hours. While most biochemical analyses, including nucleic acid detection with radioisotopes, require the ...
Control of Facial Amphiphilicity to Tune Macromolecular Interactions with BacteriaAbstract With funds provided by this NSF-ILI grant and a local match, Sinclair Community College has greatly expanded its undergraduate Organic Chemistry courses through the purchase of a Varian EM360, Mhz NMR. This acqu...
Actinobacteria are a group of Gram-positive bacteria with high guanine and cytosine content in their DNA, which can be terrestrial or aquatic. Though they are unicellular like bacteria, they do not have distinct cell wall, but they produce a mycelium that is nonseptate and more slender. Actino...
Transformationis often used to describe non-viral DNA transfer in bacteria, non‑animal eukaryotic cells, and plant cells. However, transformation also refers to a particular event or a series of events that results in a permanent change in an animal cell’s phenotype, and ...
The size and quality of the DNA was then checked on agarose gels. One microliter of pooled DNA (B15 ng) was used to amplify the 16S rRNA genes with group-specific primers targeting eubacteria (27f (Weisburg et al., 1991) and L1494 (Weisburg et al., 1991)), a-proteobacteria (a-...