The protein was purified using Glutathione Sepharose 4 Fast Flow according to the manufacturer's instructions, followed by direct cleavage of the glutathione S-transferase tag with PreScission Protease on the column. 3.3. Synthesis and labeling of the MARCKS peptide The peptide corresponding to the ...
We demonstrate the Biomedisa work flow and performance on a volumetric synchrotron X-ray microtomography (SR-µCT) dataset of a Trigonopterus weevil37,38 with a size of 1497 × 734 × 1117 voxels (Figs. 1–4 and Supplementary Movie 1). In order to create an interactive 3D rec...
for introducing solid particles into a chemical reactor comprises a flexible, cylindrical pipe through which particles flow from the top downward and is characterised in that it contains a helicoidal ramp placed inside the pipe, wound around a central axis; distance smaller than size of solid ...
The object of this two-step procedure is to avoid introducing air bubbles into the flow path. However, the requirement of flushing the buffer solution with the expensive reagent liquid results in some waste of the expensive reagent liquid as well as waste of user time. See, for example, U....