Into the Wild: Directed by Sean Penn. With Emile Hirsch, Marcia Gay Harden, William Hurt, Jena Malone. After graduating from Emory University, top student and athlete Christopher McCandless abandons his possessions, gives his entire $24,000 savings accou
I had a 'nice girl' for a senior year girlfriend. But because I could so easily get her off to myself in the car after dark, she became the object of the fantasies provoked by Playboy and Penthouse, and the Newsstand porn I hid under my bedroom dresser at home. Now you must under...
Over the first two months of the season, the Chicago Bulls have evolved into one of the better offenses in the game. Both Zach LaVine and Nikola Vucevic have boosted their value if the team chooses to move them before the February trade deadline. Unfortunately, this team has been awful on...
The data from our studies, when analyzed on an intent-to-treat basis, clearly demon- strated increased vitamin D supplementation decreased complications of pregnancy and C-section births.30,113 Further, RCT data and analysis by our group and others have clearly demonstrated that higher doses of...
Before long, we were down to the sugar cookies, which means rolling out, choices of cookie cutters, and painting. Kat, the artist, mixed the evpaporated milk and food coloring, so lots of choices there. The cookie painters take this activity seriously!
The development of mouse models of human disease and synthetic biology research by targeted transgenesis of large DNA constructs represent a significant genetic engineering hurdle. We developed an efficient, precise, single-copy integration of large tran
We tested whether constitutively active YAP2-5SA (YAP∗) (Zhao et al., 2007) gene overexpression can induce TSCs from primed hPSCs. Primed hPSCs were electroporated with YAP∗-IRES-CFP or with a control IRES-CFP construct (Figure 6A) and seeded into N2B27 medium without a TGF-β ...
dem- onstrated that OVs engineered to express the adipokine leptin boosted T cell metabolic function in the TME, and thereby allowed a superior antitumor response [15]. Den- dritic cells played important role in oncolytic virotherapy. Cytopathogenic infection of neoplastic cells releases the ...
A modified flinch–jump test was performed to assess electric footshock-evoked pain response. Footshock intensities (ranging from 0.2 to 1.0 mA) were presented through a floor bar-grid using the Freeze Monitor System (www.sandiegoinstruments.com). Rat response was rated by a blinded observer...
To confirm the identity of the autonomous CHIK replicon, we performed functional analysis of IVT-RNA from WT/eGFP. The results (Fig. S1) revealed a high eGFP-positive ratio in IVT-RNA-electroporated BHK-21 cells, indicating the successful expression of eGFP by self-amplified RNA transcripted ...