Educational language choice has been one of the most provocative issues of the 20th century and continues to be a dominant issue at the turn of the new mil
Cells were washed twice with ice-cold phosphate-buffered saline (PBS) and ruptured with RIPA buffer (Pierce, Rockford, IL) containing 5 mM EDTA, PMSF, cocktail inhibitor, and phosphatase inhibitor cocktail. Cell extracts were micro centrifuged for 20 min at 10,000×g and supernatants were colle...
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Total protein concentrations were measured using the bicinchoninic acid assay (Pierce Chemical Co Rockford, IL, USA). Samples were loaded at 40 μg/lane and separated on 8–12% SDS–polyacrylamide gels and then transferred to polyvinylidene difluoride membranes and probed with specific primary anti-...
Protein determinations were performed on the lysates using the BCA protein assay kit (Pierce Chemical Company, Rockford, IL, USA), and dilutions of the antigenic reagents used in the ELISA assays were based on protein concentration. Combinations of the above four antigenic reagents as well as T...
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7th Street- 904 7th Street, Rockford, IL 61104 Kilburn- 1219 Kilburn Avenue, Rockford, IL 61104 AboutABI Our History Organization Our History American Bentonite International, Inc (ABI)was incorporated in Illinois in 1983 to market bentonite products. Over the years, ABI has developed markets worl...
The sections were visualized as brown precipitates by adding 0.2 mg/mL 3,3′-diaminobenzidine (DAB, Pierce, Rockford, IL, USA) as a substrate. Negative control slides omitting either primary antibodies or secondary antibodies were also prepared for comparison. The slides were examined and ...
(Rockford, IL). All immunocytochemical supplies, with exception of the antibodies, were from DAKO Cytomation (Carpentaria, CA). Antibodies recognizing Rho and beta-actin as well as HRP conjugated secondary antibodies were from Santa Cruz (Camarillo, CA). Florescent staining was done with FITC ...
(Rockford, IL). TGF-β1 levels were determined with ELISA kits (Invitrogen Co.). 2.7. Statistical Analysis Comparisons of two groups used unpaired Student’s t-tests, unless an F-test showed that the variances were significantly different. When variances were significantly different, Welch’s ...