cDNA construction: The open reading frames of human LAMP-1 and human FUT9 were amplified by PCR using cDNA from HEK293T cells as a template. For the expression of FUT9 and LAMP-1, as well as its chimeric or deletion mutants (Fig. 3a), the open reading frame sequences were cloned int...
Interaction fingerprints (IFP) have been repeatedly shown to be valuable tools in virtual screening to identify novel hit compounds that can subsequently be optimized to drug candidates. As a complementary method to ligand docking, IFPs can be applied to
Miraz, M.H., Ali, M., Excell, P.S.: Adaptive user interfaces and universal usability through plasticity of user interface design. Comput. Sci. Rev. 40, 100363 (2021) Article Google Scholar Mitchell, J., Shneiderman, B.: Dynamic versus static menus: an exploratory comparison. ACM Sig...
To design peptides, the protein-protein interface of MD simulated RABV P-LC8 complex was analyzed using LIGPLOT and PPCheck server. PPCheck (http://caps.ncbs.res.in/ppcheck/) [55] ascribes pseudoenergies to protein-protein interface and Graph theoretical parameters are employed for all the ...
Template-picked particles were subjected to reference-free 2D classification and, best 2D class averages were selected and used in the second round of template picking. Improved templates allowed picking about 1.9 million particles that underwent: 2D reference-free classification and ab initio ...
7a). As shown in the cumulative amplitude plot (Fig. 7b), the curve obtained from NL2HA-S714A transfected cells was shifted to the right as compared with cells expressing NL2HA (P<0.05). Figure 7: Changes in amplitude of sIPSCs involve the interaction of Pin1 with NL2. (a) Samples ...
the protein’s CW EPR spectral amplitude from the protein alone in solution. For signal to noise reasons, we recorded the ratio of the amplitude of the immobilized component in the low field transition line and that of the central line width to plot the effect of increasing lipid ...
The E. coli CJ236 dut− ung− strain (TaKaRa, E4141S), which lacks the dUTPase and uracil N-glycosylase enzymes, was used to generate uracilated single-stranded DNA template. The E. coli TG1 electrocompetent cells (Lucigen, 60502-1) were used to produce the diversified bacterial and ph...
6), the predicted alignment error plot revealed the interaction of AlgJPp with AlgKFXPp could not be accurately predicted as the predicted alignment error values for AlgJPp were estimated to be >25 Å (Fig. S8). Thus, we were unable to generate a high-confidence model to illustrate how ...
. Obtained raw data were corrected for background fluorescence and acquisition photobleaching, normalized and further processed using Excel 2004 for Mac (Microsoft) and Systat SigmaPlot (version 10) in order to calculate recovery and regression curves as described in87....