(B) UpSet plot comparing N interactomes identified in this and six previous studies. (C) Domain organization of G3BP1 and N protein. IDR, intrinsically disordered region; RRM, RNA recognition motif; NTD, N-terminal domain; CTD, C-terminal domain. Residues 1–25 of N protein are highlighted...
cDNA construction: The open reading frames of human LAMP-1 and human FUT9 were amplified by PCR using cDNA from HEK293T cells as a template. For the expression of FUT9 and LAMP-1, as well as its chimeric or deletion mutants (Fig. 3a), the open reading frame sequences were cloned int...
Miraz, M.H., Ali, M., Excell, P.S.: Adaptive user interfaces and universal usability through plasticity of user interface design. Comput. Sci. Rev. 40, 100363 (2021) Article Google Scholar Mitchell, J., Shneiderman, B.: Dynamic versus static menus: an exploratory comparison. ACM Sig...
To design peptides, the protein-protein interface of MD simulated RABV P-LC8 complex was analyzed using LIGPLOT and PPCheck server. PPCheck (http://caps.ncbs.res.in/ppcheck/) [55] ascribes pseudoenergies to protein-protein interface and Graph theoretical parameters are employed for all the ...
Interaction fingerprints (IFP) have been repeatedly shown to be valuable tools in virtual screening to identify novel hit compounds that can subsequently be optimized to drug candidates. As a complementary method to ligand docking, IFPs can be applied to
DE and FG loops were replaced by serines in place of most of the wild-type residues to generate an inert template. A ribosome binding site coding sequence (5′-agga) was added after the HindIII restriction site and before the methionine initiation codon (ATG). The DsbA signal sequence (MK...
the protein’s CW EPR spectral amplitude from the protein alone in solution. For signal to noise reasons, we recorded the ratio of the amplitude of the immobilized component in the low field transition line and that of the central line width to plot the effect of increasing lipid ...
6), the predicted alignment error plot revealed the interaction of AlgJPp with AlgKFXPp could not be accurately predicted as the predicted alignment error values for AlgJPp were estimated to be >25 Å (Fig. S8). Thus, we were unable to generate a high-confidence model to illustrate how ...
. Obtained raw data were corrected for background fluorescence and acquisition photobleaching, normalized and further processed using Excel 2004 for Mac (Microsoft) and Systat SigmaPlot (version 10) in order to calculate recovery and regression curves as described in87....
. These results indicate that the N-terminal of β-catenin is homologous to the amphipathic helices of known interacting partners of vinculin such as α-catenin, talin, α-actinin, IpaA34,42. Thus, using α-catenin VBS from the crystal structure PDB code 4EHP23as template (Fig.2g) we ...