To remove Trx tag from Trx-galectin-8, the fusion protein was incubated with enterokinase (BioLabs, 6.4 ng per 1 mg of fusion protein) at 4 °C overnight, followed by incubation with trypsin inhibitor agarose to remove the added enterokinase. Recombinant human mature VEGF-C (21 kDa) ...
TGF-β is secreted in the tumour microenvironment in a latent, inactive form bound to latency associated protein and activated by the integrin αVsubunit. The activation of latent TGF-β by cancer-cell-expressed αVre-shapes the tumour microenvironment, and this could affect patient responses to ...
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GreenB1 has low nanomolar affinity for β1-integrin MS resulted in several additional hits besides α3- and β1-integrin and CD44 (Table S1). Because β1-integrin had a much higher signal intensity than α3-integrin in MS proteomics results, it could be the target protein within the α3...
Purified Human Integrin a5b1 Purified Protein 件 15天 会员价请登录 0 加入购物车 RH167081 CC1028 默克密理博/Millipore ADAMTS-4, Recombinant his tag 件 15天 会员价请登录 0 加入购物车 RH167082 CC1034 默克密理博/Millipore Recombinant Hu ADAMTS-5 件 15天 会员价请登录 0 加入购物车 RH167083...
Briefly, the extracellular domains of αv and β3 human integrin chains were cloned into the pBacPAK expression system, the resulting recombinant baculoviruses containing both chains were used to coinfect High Five insect cells, and the soluble receptor was harvested from the culture supernatant at ...
et al. The Integrin Specificity Of Human Recombinant Osteopontin. Biochem. Pharmacol. 58, 1567–1578, https://doi. org/10.1016/s0006-2952(99)00251-8 (1999). 30. PTeoplotimdoemllii,mAe.tiects.aEl.uMr. oJ.dMuleadt.ioCnheOmf.,α6v6β, 32-5A8 n–d26α85,βh1-ttipnst:e/g/drioni...
Fast blue Protein Stain solutionSingle-component Reagents of FLIA Kit Streptavidin-Agarose BeadsRelated products Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!) RPB282Hu01 Recombinant Integrin Alpha V (ITGaV) Positive Control; Immunogen; SDS-PAGE; WB. RPB282Hu02...
Recombinant soluble murine TGF-β receptor type II-Ig fusion protein (rsTGF-βRII-Ig) was generated as previously described7 and purchased from R&D Systems (532-R2; Minneapolis, MN). Antibodies were purchased as indicated: fluorescein isothiocyanate-conjugated pan anti-cytokeratin mAb (C-11; F3...
Fig. 1 Summary of the knottin PET ligand.aPrimary structure of lead candidate R01-MG. The engineered active loop-1 is shown in blue. The integrin avβ6core binding motif, RTDLxxL, is shown in black. The framework residues are shown in gray. Cysteine residues are shown in yellow and ...