Christine MarxDivision of Public Health Sciences, Department of Surgery, Washington University in St. Louis School of Medicine, St. Louis, MO, USAJessica VanderlanDepartment of Psychiatry, Alvin J. Siteman Cancer Center, St. Louis, MO, USADavid G. Mutch...
CHO-S cells from Life Technologies were grown in CD CHO medium supplemented with 8 mM L- Glutamine (Life Technologies) and cultivated in a 125-mL Erlenmeyer flask (Sigma- Aldrich, St. Louis, MO) with a working volume of 30 mL. The cells were incubated at 37uC, 5% CO2 with 120 rpm ...
After 14 days, cells were fixed with paraformaldehyde and stained with crystal violet (Sigma-Aldrich, St. Louis, MO). Colonies with more than 50 cells per colony were counted under a microscope. The number of colonies was quantified and compared between experimental groups, with results presented...
Thirty micromolar proteasome inhibitor MG132, 3 μM PKCθ inhibitor Rottlerin (Calbiochem, Darmstadt, Germany), 10 μg/ml ribosome inhibitor CHX (Sigma-Aldrich, St. Louis, MO, USA), 7.5 ng/ml nuclear export inhibitor leptomycin B (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and 50 ng...
Heterogeneity might come from differences in experimental designs, materials sampled, developmental stages, treatment levels (including controls), and so on. The second challenge is to develop an effective software tool to process such a large amount of data at an acceptable speed with currently ...
For genotyping, PCR analyses were performed using JumpStart™ Taq DNA polymerase and primer pairs (Sigma-Aldrich, St. Louis, MO, USA) listed in Table S3. The following PCR conditions were used: 94°C for 2 minutes 40 seconds (initial denaturation), 37 cycles of 94°C for 30 seconds (...
(Sigma, St. Louis, MO) and, in some cases, 25 mM QX-314 dissolved in 1 M potassiumacetate (Sigma)] were lowered into the cortex (as far as possible) directly contralateral to the stimulating electrode. When a neuron was im- paled, stimuli were presented with increasing amplitude (n 10...
The following primary antibodies were obtained from the indicated sources: monoclonal antibodies (mAbs) against tubulin (clone B-5-1-2) and against the FLAG epitope (clone M2), Sigma-Aldrich (St Louis, MO); anti-PDI mAbs (clone 1D3), Merck-Millipore (Burlington, MA); anti-GFP polyclonals...
Stable isotope-resolved metabolomics (SIRM) provides information regarding the relative activity of numerous metabolic pathways and the contribution of nutrients to specific metabolite pools; however, SIRM experiments can be difficult to execute, and dat
An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5′ and 3′ ends) at the ETIP genomic location. One element in the...