Nanjing, China). Then, StepOne Real-Time PCR System (Applied Biosystems, Carlsbad, USA) and the 2× M5 HiPer Realtime PCR Super mix (Mei5bio, Beijing, China) were used for following reactions
Parylene deposition is carried out with a Model 2010 system (Specialty Coating Systems, Indianapolis, IN, USA). The completely assembled device can be autoclaved and stored for use in ambient conditions, similar to a commercial transwell. Sensor-integrated cell culture housing The transwell housing ...
Briefly, genomic DNA of the bacterial strains was isolated from an overnight culture using PureLink® Genomic DNA Mini Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and sequenced by SNPsaurus (Eugene, OR, USA) using an Illumina HiSeq 2000 platform, generating 2 × 150-bp paired-end ...
The Qubit® dsDNA Assay Kit in the Qubit® 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) was used to measure DNA concentration (μg/mL). The NEBNext® Ultra™ DNA Library Prep Kit was used according to the manufacturer’s recommendations to generate sequencing libraries. Index...