001006282 成立日期: 2009-06-15 企查查编码: QUS1H39XC8 办公地址: 1710 COMMERCIAL PARK CORALVILLE,IA 52401 USA 基本信息 企业注册号 001006282 企业名称 INTEGRATED DNA TECHNOLOGIES, INC. 成立日期 2009-06-15 企业类型 - 注册地 MASSACHUSETTS(马萨诸塞州) ...
Coralville, IA, USA) were annealed in Integrated DNA Technologies Duplex Buffer using a thermal cycler with a 1-µg concentration of the guide RNA. The guide RNA and Cas9 v3 protein (Integrated DNA Technologies) were assembled in water at room temperature (50 ng/µl each). Water and th...
Primers were ordered from Integrated DNA Technologies (Coralville, IA) and listed in Supplementary Data 2. Plasmid pSkunk3-BLA was purchased from Addgene (plasmid 61531). PacBio Barcoded Universal Primers (Part Number: 101-629-100) was purchased from Pacific Biosciences (Menlo Park, CA). ...
INTEGRATED DNA TECHNOLOGIES, INC. 1710 COMMERCIAL PARKCORALVILLE, IA 52241-2760 17 $144,410 INTEGRATED DOCUMENT SYSTEMS IN 7500 E ARAPAHOE RD STE 250ENGLEWOOD, CO 80112 1 $33,914 INTEGRATED DOOR SOLUTIONS, INC 2298 MANHATTAN PLSANTA CLARA, CA 95051-1736 1 $10,599 INTEGRATED DYNAMICS ENGINEE...
ESM and ASM female pupae (n = 5/sampling point/strain) were homogenized individually in Trizol reagent (Ambion, Life Technologies; 4 to 7 mL depending on the size of the pupa) using 15 mL disposable tissue grinders (VWR, Cat.47732–446). They were then flash frozen in liquid nitroge...
( ≥ −12 kcal mol−1). Oligonucleotides were prepared using standard desalting by Integrated DNA Technologies (Coralville, IA, USA). Recombineering was performed in the electrocompetentE. coliK-12 BW25113 cells carrying pORTMAGE3 (Addgene plasmid ID: 72678). pORTMAGE3 was induced...
The primer and probe oligos were designed using PrimerQuest Tool (Integrated DNA Technologies, Coralville, IA). All primers were checked for target sequence specificity using NCBI Primer-BLAST44and UCSC In-Silico PCR (https://genome.ucsc.edu/, last accessed May 27, 2020) using default settings...
(mPEG-SH) was purchased from Nanocs. Magnetic beads were purchased from Life Technologies. All DNA sequences (TableS1) were synthesized by Integrated DNA Technologies (IDT, Coralville, IA). Millipore Synergy ultrapure water (DI) of resistivity = 18.2 MΩ cm was used in all nanoparticle ...
The reagent composition included 4 µL of a home recipe LAMP buffer and 2 µL of primers (Integrated DNA Technologies, Coralville, IA, USA). The forward outer primer sequence was CCAACAGTATATAGTGCAACTTC, and the backward outer primer sequence was TTGCATTTTCTACCATTTTTTTCG at a final ...