Cassette mobility in Escherichia coli was detected by excision/integration into the recipient integron In3 on the conjugative plasmid R388 with the overproduced integrase. Despite several antibiotic pressures, the bel-1 cassette remained at the second position in the integron, highlighting its stability...
The integrated conjugative plasmid pSAM2 of Streptomyces ambofaciens is related to temperate bacteriophages. The integration event is promoted by a site-specific recombinase of the integrase family. The int gene encoding this integrase is closely linked to the ... F Boccard,T Smokvina,JL Pernodet...
Also the so-called master activator of IncA/C conjugative plasmids AcaDC can induce excision and transfer of different classes of chromosomal mobile genetic elements such as the Salmonella enterica genomic island 1 (SGI1) and the MGIVmi1 of Vibrio mimicus (Carraro et al., 2014). Recently, ...
A plasmid containing two oriT copies resident in the recipient cell undergoes recombination when a TrwC-piloted DNA is conjugatively transferred into it. Finally, we show TrwC-dependent integration of the transferred DNA into a resident oriT copy in the recipient cell. Our results indicate that ...
1994. Conjugative transposition: Tn916 integrase contains two independent DNA binding domains that recognize different DNA sequences. EMBO J. 13:1541-1548.Lu and Churchward (1994) " Conjugative transposition: Tn916 integrase contains two independent DNA binding domains that recognize different DNA ...
Integrative and conjugative elements (ICEs) are chromosomally-integrated mobile genetic elements that excise from their host chromosome and transfer to other bacteria via conjugation. ICEMlSymR7A is the prototypical member of a large family of "symbiosis ICEs" which confer upon their hosts the ...
IntDOT is an enzyme required for the recombination reactions that promote movement of CTnDOT that is an integrative conjugative element (ICE) found in Bacteroides spp. IntDOT has been classified in the tyrosine recombinase family. However, unlike most other tyrosine recombinases that require sequence...
coli CC118λpir and S17.1λpir were used as a host for replication and as a conjugative strain, respectively. Plasmid single cross-over insertion into Acinetobacter was selected by gentamicin and the double cross over by plating on 6% sucrose containing to check for the loss of the levan...