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Manual injection, oven temperature of 60 °C, injector temperature of 120 °C, TCD temperature of 130 °C, and carrier gas pressure of 0.04 MPa were employed in conjunction with a PorpakN packed stainless steel column and ultra-high quality helium carrier gas for the separation [21]. 2.3....
Library preparation for sequencing was performed using a Nextera XT DNA Library Prep Kit (Illumina, USA) according to the manufacturer’s preparation manual. ASFV genome sequencing An Illumina MiSeq machine was used for the sequencing. Briefly, the genomic library was normalized following the manufac...
Ion, ligand placement and manual building of the structure was done in Coot [37]. Structure validation was performed using MolProbity [38]. Molecular graphics were prepared using PyMOL [39]. Superimposition of the structures and calculation of the root mean square deviation (r.m.s.d.) ...
303p. $59.95 hardbound (ISBN 978-0-7890-2800-6/0-7890-2800-X); $39.95 softbound (ISBN 978-0-7890-2801-3/0-7890-2801-8). Published simultaneously as Cataloging and Classification Quarterly 40, nos. 3/4. Metadata: A Cataloger's Primer is edited by Richard Smiraglia, a noted expert ...
Integrated Raman intensities of pure water were also obtained in the temperature range of 10°—90°C for the intramolecular valence and deformation contours in the spectral region of 2800–3900 cm1, and near 1645 cm1, respectively. The integrated intensity of the deformation...