Position-specific insertion vectors and method of using same : Sandmeyer University of California US 5482853; January 9, 1996doi:10.1016/S0141-0229(97)81304-XEnzyme & Microbial Technology
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Ideally, therapeutic transgenes of choice could be stably introduced to the human genome at a safe-harbor locus. Safe-harbor genome insertion has been favored in eukaryotic evolution by site-specific retroelements3. Some non-long terminal repeat (non-LTR) retroelements show exquisite insertion-site...
Transposon-inserted sites, insert orientation, in-frame insertions and their read count were identified by FASTQINS31 looking for library-specific IRs (see Methods; Supplementary Data 2 and 3). For each sample, referred as ReporterTypeConcentration (e.g. CmB15 corresponds to CmB grown with 15...
Rate limiting of the service (will probably be provided in the access network using the OLT or ONT capabilities or via a BRAS or specific rate shaping device at a higher point in the network); ▪ Tracking usage and metrics of the service (such as implementation with a BRAS or via SNMP...
advantageous compared with the AOS insertion technique.Conclusion. This finding leads to the conclusion that, in general, consideration of the specific curling behaviour of a CI electrode array is beneficial in terms of less traumatic insertion. Therefore, these results highlight an entirely novel ...
(CRISPR)/Cas systems are a dominant defense mechanism that protect prokaryotes against invasive genetic elements through the use of specific guide RNAs (crRNAs) generated from the CRISPR array, a bank of DNA sequences inserted in the host genome and derived from foreign genetic material5,6. ...
(GenBank accession numbers: chromosome: NC_004347.2; megaplasmid: NC_004349.1). The algorithm builds a lookup table indexed by possible insertion location, records the locus name in which this position falls and marks the location as either ‘Dispensable’, ‘SurpriseDispensable’, ‘GuessDispensable...
In order to achieve this electrode array position close to the inside wall of the cochlea, the electrode needs to be designed in such a way that it assumes this position upon or immediately following insertion into the cochlea. This is a challenge as the array needs to be shaped such that...
With this modification, the transposases can be applied in a similar fashion as site specific recombinases such as cre and flp. However, in contrast to the above-mentioned recombinases, integration occurs in the vicinity of the site but not at the exact position of the selected site providing...