They then tested inserting end-modified DNA fragments of varying sizes at multiple points in the genome, using CRISPR-Cas9 to precisely target specific sites for insertion. The researchers found that by chemically tweaking the ends of the DNA to be inserted, the new technique is two to five ti...
Modulating DNA Repair Pathways to Improve Precision Genome Engineering Programmable nucleases like the popular CRISPR/Cas KS Pawelczak,NS Gavande,PS Vandervere-Carozza,... - 《Acs Chemical Biology》 被引量: 5发表: 2018年 CRISPR-Cas Genome Surgery in Ophthalmology Genetic disease affecting vision ...
The IBM workflow is modular in design and switching to another intein should be as simple as employing a new substitution DNA fragment during the step of transposon replacement in Fig.1a. To demonstrate this, we employed the gp41-1 intein34with the −2, −1 and +1, +2 minimal extein...