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Full size image Fsx1 is a structural homolog of HAP2/GCS1 To experimentally investigate the presence of fusexin-like proteins in Archaea, a selection of the candidate genes was expressed in mammalian cells (Supplementary Fig. 1b, c). High-level expression was observed for a metagenomic Fsx1...
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Telomere crisis is linked with many of the genomic alterations found in cancer genomes. A new understanding of how these alterations arise points towards an active role for innate immune sensors during crisis and to new opportunities for the treatment an
Instant access to full article PDF Buy now Additional access options: Log in Learn about institutional subscriptions Read our FAQs Contact customer support References Download references Acknowledgements We are grateful to B. Diaz, M. Buschman and C. Gould for reading the manuscript and to J. Tsai...
Full size image The production of pamamycins 663 by S. albus P21pamW R2-73 and R2-100 was 9 and 8 times higher than in the case of the same host carrying the R2 cosmid (Fig. 4). The accumulation of other pamamycins was also affected in the recombinant strains with R2-73 and R2...
To better understand the mechanistic basis of the genetic observations described above, we purified full-length Bud14 from E. coli (Figure 4A) and tested its in vitro effects on formin-mediated actin assembly. Bud14 had no effect on Bni1(FH1-FH2-C) activity, but potently inhibited Bnr1(...
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Full size image A second pathway to PG degradation, designated as the pinholin-SAR endolysin paradigm, has recently been described in detail, using the lambdoid phage ϕ21 (Fig.1a) as a model [4]. In this pathway, the muralytic enzyme, R21, is secreted through the host translocon and...
Full size image We detected in both annelid and mollusk genomes Met RTKs, with a domain structure like human Mets, consisting of a truncated plexin-like extracellular domain with the Sema plus PSI domain, followed by four IPT domains, and an intracellular tyrosine kinase domain (Fig.3A,B)....