This method doesn't appear to work with BL21,so just grow the cells at 30 or 37 ℃ when making BL21 competent cells.However,it has been suggested that the efficiency of BL21 prepared using Inoue method may be improved by treating it with DTT before freezing (add to 3.5% v/v of a 2.2...
Freezing the cells appear to make cells more competent. Some cell strains may work better than others (DH5alpha works well in my hand).Note also that some cells (e.g.HB101)has greater recombination activity than others. This method doesnt appear to work with BL21,so just grow the cells ...
This method doesn't appear to work with BL21,so just grow the cells at 30 or 37℃when making BL21 competent cells.However,it has been suggested that the efficiency of BL21 prepared using Inoue method may be improved by treating it with DTT before freezing (add to 3.5% v/v of a 2.2M...
This method doesn't appear to work with BL21,so just grow the cells at 30 or 37 ℃ when making BL21 competent cells.However,it has been suggested that the efficiency of BL21 prepared using Inoue method may be improved by treating it with DTT before freezing (add to 3.5% v/v of ...
无菌操作要好其他的注意事项都不知道，现已制备好超感细胞，后来网上发现一句话“This method doesn't ...
Freezing the cells appear to make cells more competent. Some cell strains may work better than others (DH5alpha works well in my hand).Note also that some cells (e.g.HB101)has greater recombination activity than others. This method doesn't appear to work with BL21,so just grow the cells...