metabolic labeling of cells with radioactive [3H]myo-inositol or [32P]orthophosphate has been the most widely used technique in the field (Menniti, Miller, Putney, & Shears, 1993;Stephens et al., 1993). The resulting radioactively labeled InsPs can be isolated byperchloric acidextraction of ...
To elucidate the biological functions of these molecules, robust techniques to characterize inositol phosphate metabolism at the cellular level are highly sought after. This chapter provides a detailed protocol for the preparation of 13C-labeled myo-inositol, its use for metabolic labeling of mammalian...
Inositol is released from phytic acid through the digestive process.Probiotic lactobacilli, and other species of the endogenous digestive microflora as well, are an important source of the enzyme phytase which catalyses the release of phosphate, mineerals and trace minerals, rendering them more ...
both activated by the TSH and its receptor (TSHR) binding. The cAMP cascade regulates thyrocytes development and differentiation and TH secretion, while the PLC-dependent inositol phosphate Ca2+/DAG pathway results in enhanced H2O2 production, ...
the promoters of key metabolic genes involved in glycolysis and pentose phosphate pathways, includingzwf,pgiandpfk1, were replaced with the glycerol-inducible promoter pGUT1. Therefore, dynamic regulation of cell growth and inositol production was achieved via supplementing glycerol and glucose sequentiall...
process in the vessel wall microenvironment, acting to promote or inhibit calcification in conjunction with local precipitation of calcium and phosphate8. The most important endogenous calcification inhibitors include serum fetuin-A14, matrix Gla protein15, and pyrophosphate (PPi)16. Most drug candidates...
In particular, lithium inhibition of inositol monophospha- tase was shown in cell culture to increase IP1 (inositol phosphate-1) concentrations which in turn mediated the rate of phosphoinositide synthesis [7]. We reasoned that a loss-of-function mutation of IMPA1 may result in a similar ...
Here we briefly summarise the parts of the inositol phosphate metabolic network important to our argument. Historically, inositol phosphates acquired substantial research interest after the discovery that the hydrolysis of the lipid PI(4,5)P2 by PLC generates two second messengers: diacylglycerol (DAG)...
KenStuartSDOSCell Chemical BiologyCestari, I., Haas, P., Moretti, N.S., Schenkman, S., and Stuart, K. (2016). Chemogenetic Characterization of Inositol Phosphate Metabolic Pathway Reveals Druggable Enzymes for Targeting Kinetoplastid Parasites. Cell Chem Biol 23, 608- 17....
1.An IPL compound comprising phosphatidyl-myo-inositol-phosphate (PIPn) with an absolute stereochemical structure of 1D-1-(1,2-di-O-fattyacyl-sn-glycero-3-phospho)-myo-inositol, wherein either the group at both the sn-glycero-1- and the sn-glycero-2-positions is isostearoyl, or one of...