The weaker effect ofhrp1Δ on CENP-ACnp1chromatin suggests that Chd1Hrp1may complement the function of the Ino80 complex. We were unable to generateies6Δhrp1Δ cells, suggesting thaties6Δ andhrp1Δ are synthetically lethal. Theies2Δhrp1Δ double-mutant cells were viable, and showed lar...
Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling. Journal of Biological Chemistry. 286, (13), 11283-11289 (2011). Sauer, B. Site-specific recombination: developments and applications. 5, (5), ...
Different parts of the SWR1 complex have been shown to interact with histone H2A.Z–H2B dimers and to facilitate/regulate their exchange15,46. The Swc2 subunit of SWR1 has histone chaperone-like activity that stimulates the incorporation of H2A.Z22,46,47. Interactions of SWR1 with the H2A...
Stalling of RNA Polymerase II (RNAPII) on chromatin during transcriptional stress results in polyubiquitination and degradation of the largest subunit of RNAPII, Rpb1, by the ubiquitin proteasome system (UPS). Here, we report that the ATP-dependent chromatin remodeling complex INO80 is required for...
Instead, Rvbp are essential for the complete assembly of a functional Ino80 complex, nucleating the association of the actin-like Arp5p subunit with the rest of the complex in an ATP-dependent reaction. Results Acute Removal of Ino80p or Rvb2p Reveals Considerable Overlap in Effects on ...
To define the location of subunit modules that interact with the N-terminal domains of the ATPase subunits, INO80-C was purified from an arp8D strain. The resulting complex lacks the entire Arp8/Arp4/Act1 module that interacts with the Ino80 HSA NATURE COMMUNICATIONS | 6:7108 | DOI: ...
Subunit Rtt102 controls the conformation of the Arp7/9 heterodimer and its interactions with nucleotide and the catalytic subunit of SWI/SNF remodelers. J. Biol. Chem. (2013). Aramayo, R. J. et al. Cryo-EM structures of the human INO80 chromatin-remodeling complex. Nat. Struct. Mol. ...
We deleted the N-terminus of Arp8 to determine if loss of this region alone disrupts complex integrity, reduces the enzymatic activity of INO80, or binding of the Arp8 module to linker DNA. Whereas deletion of the entire Arp8 subunit causes a loss of Arp4 and actin from the INO80 compl...