Relevant structures are labeled. (B) Higher magnification image of the stria vascularis from (A) showing a normal distribution of basal (b), intermediate (i), and marginal (m) cell nuclei and a normal sized blood vessel (arrow). (C) Higher magnification image of the organ of Corti in (...
To realize the envisioned benefits for clinical purposes and fix the pitfalls of the manual workflow, the two related tasks of a) semantic segmentation of inner ear structures and b) localization of landmarks have to be solved by a fully automated pipeline with minimal user interaction. Success ...
Study Design: Mouse inner ear specimens were fixed with minimal dissection, rapidly decalcified and fluorescently labeled by immunohistochemistry then impregnated by epoxy resin or a clearing agent composed of 5 parts methyl salicylate:3 parts benzyl benzoate. The specimens were imaged by both confocal...
Preservation of the slides for documentation was improved by fixing the intermediate layer of the antigen and the labeled antibdoy in cold ethanol. The inner ear tissue preparations retained their fluorescent staining up to 6 months. Our method is convenient for screening patients with inner ear ...
5G–I). We also examined the number of neurons labeled after lipophilic dye injection into the ear and found ∼ 30 small neurons scattered along the nerve fibers reaching toward the brain. Only an occasional efferent fiber was found at this late stage (E17.5) to reach to the inner ear ...
Nuclei were labeled with DAPI. The specimens were viewed with 63× oil objective. Scale bar = 200 μm. Full size image VEGF increase due to EP2 and EP4 agonists To examine the changes in the expression of VEGF proteins and VEGF mRNAs in the inner ear caused by EP2 and EP4 agonists, ...
GFP-labeled hair cells were isolated from inner-ear sensory epithelia of a pou4f3 promoter-driven GAP-GFP line of transgenic zebrafish. One thousand hair cells and 1,000 non-sensory surrounding cells (nsSCs) were separately collected for each biological replicate, using the suction pipette ...
Grxcr1 is expressed in sensory epithelia of the inner ear, and its encoded protein is localized along the length of stereocilia, the actin-filament-rich mechanosensory structures at the apical surface of auditory and vestibular hair cells. The precise architecture of hair cell stereocilia is ...
2C). This indicates that proteins in the axon and the inner tongue structures were turned over faster compared to the compact myelin sheath. Similar results were obtained from an iKO mouse pulse-labeled for 60 days (Fig. S7). Fig. 2: Determination of myelin turnover by 13C-lysine feeding...
Methods for introducing a gene into a cell of the inner ear, e.g., a cochlear or vestibular cell, e.g., a hair cell, e.g., for therapy, that include the use of exosomes associated w